高效液相色谱法在甘油含量测定中应用的研究

Application of HPLC Method in Determining Content of Glycerol

现有的甘油含量测定标准化方法，都是以假设“无杂质”为基础的。该法与气相色谱法，均以分离色谱为基础，解决了滴定法不具分离鉴别的缺点。完善了原气相色谱法在不同含量规格适用范围上的局限性，使液相色谱法对不同含量规格甘油定量测试的方法标准化成为可能。该法与气相色谱的“杂质倒扣”法的结合应用和互补，使定性鉴别和不同规格甘油含量测定得以完整组合。其检测下限：44．46ng／μL，线性相关系数r=0．99999，回收率：98．03％～99．88％，重复性：-↑Sr=0．4018，-↑r95=1．125，再现性：-↑SR=0．4463，-↑R95=1．250。

Current standard methods for determining the content of glycerol are all based on assuming the determined glycerol to have ＂no impurity＂ except water. Themethods of HPLC and GC in glycerol determination are all based on chromatographic fractionation. Therefore, they can overcome the defects of chemical titration method on qualitative identification and impurity separation. This HPLC method will further perfect the limitation that previously existed in GC standard method on the applicable range of different content specifications, and make it possible to standardize the test method for the different content specifications of glycerol by HPLC method. It will be a complete combination for qualitative identification and quantitative analysis for the different content specifications of glycerol by the combined application and mutual complement of these HPLC andtGC methods. For this method, its＇required detection limit is 44.46 ng/μL, its＇ coefficient is 0. 99999, its＇ recoveries are 98.03% - 99.88%, its＇ repeatabilities （r） are： -↑Sr = 0.4018, -↑r95 = 1.125, and its＇reproducibilities （R） are： -↑SR = 0.4463,-↑ R95 = 1.250.