摘要
建立了一种HPLC法测定氢碘酸水苏碱的含量。样品用C18柱分离，流动相为甲醇：0．1％磷酸=9：1（v：v），检测波长220nm，流速1．0mL／min。氢碘酸水苏碱在1.8μg范围内呈良好线性关系，r=0．9998。平均回收率为98．64％，RSD=0．38％。该法准确、快速、简单，可作为测定氢碘酸水苏碱含量的方法之一。
A HPLC method for quantitative determination of stachydrine hydroiodic was established. The sample was separated by using column C18, and the mobile phase used was MeOH- 0. 1% H3PO4 （9： 1 ）. The detection wavelength was set at 220 nm, and the /low rate was 1.0 mL/min. The linear range was 1-10 μg, r = 0.9998. The average recovery-rate was 98.64 %, RSD = 0.38 %. This method is accurate, rapid and simple, and it can be used for the quality control of sfachydrine hydroiodic.
出处
《化学世界》
CAS
CSCD
北大核心
2007年第11期650-651,677,683,共4页
Chemical World
基金
安徽省教育厅自然科学基金资助(KJ2007B364ZC)
关键词
氢碘酸水苏碱
HPLC
含量测定
stachydrine hydroiodic
HPLC
quantitative determination
