摘要
病程相关蛋白基因PR-1、PR-2和PR-5是植物抗病基因介导的抗病反应和系统获得抗性(systemic acquired resistance,SAR)中的标志基因。为了研究病程相关蛋白基因和水杨酸在小麦(TriticumaestivumL.)抗白粉病反应中所起的作用,以抗白粉病和感白粉病小麦品系为材料,在白粉病菌(Blumeria graminisf.sp.tritici,Bgt)诱导不同时间,或水杨酸处理不同时间后,用半定量RT-PCR技术检测了小麦叶片中PR-1、PR-2和PR-5基因的表达变化。结果表明,白粉病菌的侵染激活和显著增强了病程相关蛋白基因PR-1、PR-2和PR-5在周麦18中的转录。与感病品系相比,PR-1和PR-5在抗病品系中转录激活得更快、更强。水杨酸处理显著激活了PR-1和PR-5的转录,但对PR-2的转录影响不大。白粉病菌和水杨酸均能显著激活和增强PR-1和PR-5的表达,但白粉病菌的作用更强,说明PR-1和PR-5基因在小麦抗白粉病反应中起重要作用。PR-1和PR-5可以作为小麦SAR的标志基因。水杨酸处理后周麦18和中国春的白粉病抗性得到提高,提示水杨酸在小麦抗白粉病信号传导途径中起一定作用。
Pathogenesis-related protein genes(PRs) of PR-1,PR-2 and PR-5 are the marker genes of plant disease resistance(R) gene-mediated resistance and systemic acquired resistance(SAR).To investigate the roles of PR genes and salicylic acid(SA) in wheat(Triticum aestivum L.) powdery mildew resistance reaction,the expression patterns of PR-1,PR-2 and PR-5 in seedling leaves of susceptible and resistant wheat cultivars or lines were analyzed by semi-quantitative RT-PCR after Blumeria graminis f.sp.tritici(Bgt) infection or SA treatment.The results showed that the transcribing of PR-1,PR-2 or PR-5 was significantly enhanced by Bgt infection in Zhoumai 18.The expressions of PR-1 and PR-5 were activated more quickly and strongly in resistant wheat than in susceptible wheat.SA significantly activated the transcriptions of PR-1 and PR-5,but it had less effect on PR-2.Both Bgt and SA could significantly upregulate the expressions of PR-1 and PR-5,but the effect of Bgt was stronger than that of SA.Therefore,PR-1 and PR-5 played important roles in wheat powdery mildew resistance.PR-1 and PR-5 may be considered as the marker genes for SAR in wheat.With a role in wheat powdery mildew resistant signal transduction cascades,SA application increased the powdery mildew resistance of Zhoumai 18 and Chinese Spring.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2007年第6期1132-1137,共6页
Journal of Triticeae Crops
基金
This project was supported by The Innovation Fund for Outstanding Scholar of Henan Province(0621001700)
the Tackle Key Problem Project in Henan(0523010700)
The Innovation Fund of Henan Agricultural University (2006).
关键词
小麦
白粉病
水杨酸
病程相关蛋白基因的表达
Wheat,Resistance to powdery mildew,Salicylic acid(SA),expression of Pathogenesis-related protein gene(PR)