长梗秦艽的化学成分

Chemical Constituents of Gentiana waltonii

目的：研究长梗秦艽（Gentiana waltoniiBurkill）的化学成分,为阐明其药效物质奠定基础。方法：通过大孔树脂、硅胶、sephadex LH-20、反相硅胶（Rp-18）柱层析及制备液相色谱（Prep LC）进行分离,根据化合物理化性质和光谱数据鉴定结构。结果：从该植物中分离鉴定了10个化合物,分别为：gentiolactone（1）、2-methoxy-anofinic acid（2）、4,6-二羟基-2-甲氧基苯乙酮（4,6-dihydroxy-2-methoxyacetophenone,3）、东莨菪素（scopoletin,4）、6′-（2,3-二羟基苯甲酰）当药苷酯（6′-（2,3-dihydroxybenzoyl）sweroside,5）、3′-乙酰基当药苷（3′-acetylsweroside,6）、4-O-2-羟基-6-甲氧基苯乙酮苷（4-O-glycosyloxy-2-hydroxy-6-methoxyacetophenone,7）、獐牙菜苦苷（swertiamarin,8）、当药苷（sweroside,9）和龙胆苦苷（gentiopicroside,10）。结论：化合物3、4和7为首次从龙胆属植物中分离得到,化合物1~10均为首次从该植物中分离得到。

AIM： To evaluate the chemical constituents in Gentiana waltonii Burkill. METHODS： Isolation and purification of the compounds were carried out by column chromatography with maernporous resin, silica gel, Sephadex LH-20, Rp-18 silica gel and preparative HPLC. Struetural identification of eompotunds was based on spectral data analysis. RESULTS： Ten compounds were obtained from Gentiana waltonii and identified as gentiolactone （1）, 2-methoxyannfinic acid （2）, 4, 6-dihychroxy-2-methoxyacetophenone （3）, scopoletin（4）. 6′-（2, 3-dihydroxybenzoyl） sweroside （5）, 3′-aeetylsweroside （6）, 4-O-glycosyloxy-2-hydroxy-6-methoxyacetophenone （7）, swertianmarin（8）, sweroside （9） and gentiopicroside （10）, CONCLUSION： Compounds 3, 4, and 7 were isolated from the genus Gentiana for the first lime. Compounds 1 ～ 10 were isolated from this planl for the first lime.