N-CWS中多糖的促进小鼠脾淋巴细胞增殖作用

Effects of Polysaccharide from Nocardia rubra Cell Wall Skeleton on the Proliferation of Mouse Splenic Lymphocytes

目的:从红色诺卡氏放线菌细胞壁骨架(N-CWS)中分离筛选新的免疫活性组分。方法:MTT法检测N-CWS中的主要组分对脾淋巴细胞增殖的影响,并对活性组分进行气相色谱分析。结果:在4.5～144μg.mL-1范围内,N-CWS中的多糖(PS)具有明显的刺激脾淋巴细胞增殖的生物活性,并在9～18μg.mL-1时刺激作用最强;它是一种主要由阿拉伯糖和半乳糖按照1∶1.66的摩尔比组成的阿拉伯半乳聚糖。结论:N-CWS的抗肿瘤和免疫调节活性主要与其多糖类成分有关。

AIM： To find new immunological active compositions from Nocardia rubra cell wall skeleton（N-CWS）. METHODS： The componenls from N-CWS were purified, and their activity on the growth of mouse splenic lymphocyles was analyzed by MTT method. The chemical analysis of active composition was made by GC. RESULTS： The polysaccharide（PS）could stiulate the proliferalion of mouse splenic lymphocytes apparently at the coneentration of 4.5-144μg· mL ^-1 and exert its effects at the concentration of 9-18 μg· mL^-1 markedly. GC investigation confirmed thai the polysacchauide was a kind of arabinogalaclan in which the arabinose / galaclose rate was about 1.66. CONCLUSION： The immunological activity of N-CWS on Ihe growth of tumor cells is mainly related to its polysaccharide.