大鼠脑突触体的制备及形态和活性研究

Perparation of synaptosomes from rat brain and studies of theirmorphology and activity

用低浓度（０．３及０．８ｍｏｌ／Ｌ）蔗糖熔液为分离介质，在低离心力下制备各大鼠脑突触体。以电镜观察和测定乳酸脱氢酶活性检测其结构的完整性，以高钾刺激下突触体内游离Ｃａ＋浓度和氨基酸《Ａｓｐ，Ｇｌｕ，Ｇｌｙ，Ｔａｌｌ和ＧＡＢＡ）释放量的变化判断其生物活性。结果表明，本方法制备的突触体具有结构完整性和较强的生物活性。本实验建立了一个快速制备突触体的方法。

Synaptosomes were prepared with sucrose solution of low concentration(0.3 and 0.8mol/L) at relatively lowcentrifugal forces. The morphology of synatosomes was investigated by electron microscope and by thedetermination of the activity of lactate dehydrogenase in and out of synaptosomes .The intrasynaptosomal freecalcium concentrations and the release of amino acids (Asp, Glu, Gly, Tau, GABA) from them in resting anddepolarised conditions were also determinded in order to observe the biogical activity of the synaptosomesstate The present study has established a rapid and useful method for preparing synaptosomes.