热毒宁注射液对急性肺损伤模型兔肺表面活性物质的影响

Effect of Reduning Injection on the Surfactant of the Rabbit Lung Subjected to Acute Injury

目的观察热毒宁注射液(RDN)对于内毒素性急性肺损伤(ALI)兔肺表面活性物质的影响。方法健康兔30只,随机分3组,每组10只,即对照组、模型组和RDN治疗组。模型组和RDN治疗组通过静脉注射内毒素建立急性肺损伤的模型。RDN治疗组在不同时间静脉注射RDN 5 mL.kg-1,不同时间点检测外周血液白细胞介素-8(IL-8)、IL-10、肿瘤坏死因子(TNF)-α的含量及支气管肺泡灌洗液(BALF)内的表面活性物质(主要成分为磷脂酰胆碱,PC)和磷脂酰乙醇胺(PE)的含量。结果内毒素诱导的兔ALI中,动物血和BALF中的致炎因子含量较对照组显著升高,肺通透指数升高,PC下降,PE增加(P<0.05)。而RDN的使用均可抑制上述各变化(P<0.05)。结论RDN可提升ALI兔的肺表面活性物质的含量,抑制ALI的发生与发展,对ALI有一定的治疗作用。

Objective To Study the effect of reduning injection（RDN） on the surfactant of the rabbit lung subjected to acute injury（ALI） induced by endotoxin（LPS）. Methods 30 healthy rabbits were randomly divided into three equal groups： the control group, model group and RDN treatment group. A model of acute lung injury was set up in each of the animals of the model group and RDN treatment group by IV injections of endotoxin（LPS）. Rabbits of the RDN treatment group were given each an IV injection of 5 mL · kg^-1 of RDN for three times after the injection of LPS. At different time points following the RDN treatment, blood samples were collected for the content determination of IL-8, IL-10 and TNF-α while bronchoalveolar lavage fluid （BALF） was harvested for the determination of the content of surfactant the major constituents of which were phosphatidyl choline （PC） and phosphatidyl ethanolamine （PE）. Results In rabbits of the model group, the contents of the pro-inflammatory eytokines in the arterial blood and BALF were strikingly higher than those in the specimens from animals of the control group. There was also an increase in the pulmonary permeablity index as well as a decrease in PC and an increase in PE（ P 〈 0.05 ） in the BALF from animals of the model group. Administration of RDN was shown to mitigate the above-mentioned changes. Conclusion RDN was shown to increase the surfaetant of the rabbit lung subjected to acute LPS-indueed injury leading to an inhibition of the occurrence and development of the lung injury.