邻二氮菲分光光度法测定鸡血藤 片姜黄 降香3种中药中铁的含量

Determination of the Iron in the Caulis Spatholobi,Rhizoma Curcumae Longe,Lignum Dalbergiae Odoriferae by Spectrophotometry

用邻二氮菲分光光度法测定了鸡血藤、片姜黄、降香3种中药微量元素铁的含量。将中药消化后配制成溶液。在pH=4～6下，用盐酸羟胺将三价铁还原成二价铁。二价铁再与邻二氮菲生成桔红色络合物。在波长510nm处用分光光度法来测定各样品的吸光度A，求出样品中微量元素铁的含量。结果显示：3种中药铁含量丰富，其中铁的含量由高到低顺序为：片姜黄〉降香〉鸡血藤。测定结果为进一步研究鸡血藤、片姜黄、降香3种中药的药物功效作用提供了可靠的数据，为中药开发和应用提供了科学的依据。

The content of iron in Caulis Spatholobi, Rhizoma Curcumae Longe, Lignum Dalbergiae Odoriferae was determined by Spectrophotometry. The samples were digested that in pH 4 - 6, the Fe^3＋ was deoxidized to Fe^2＋ by hydroxylamine hydroehlorlde, then the Fe^2＋ and 1,10- phenanthroline produced a nacaratcomplexes. The iron content of the sample was calculated by measuring the absorbances of sample at 510 nm by spectrophotometer. The results show that there are considerably rich iron in Chinese herbs. The order： Lignum Dalbergiae Odoriferae more than Rhizoma Curcumae Longe; Rhizoma Curcumae Longe more than Caulis Spatholobi, It concludes that the rusults give some reliable data for studying the medicinal role？ of Caulis Spatholobi, Rhizoma Curcumae Longe, Lignum Dalbergiae Odoriferae, which can provide some scientific data for developing and making use of chinese herbs