小鼠植入前胚胎MuERV-L基因表达特征研究

Expression of MuERV-L mRNA in the preimplantation mouse development

利用实时定量RT-PCR技术,对体外培养的小鼠植入前胚胎发育过程中鼠内源逆转录病毒样基因（MuERV-L）的表达进行研究,并通过appd icolin（DNA复制抑制剂）、TSA（组蛋白去乙酰化酶特异性抑制剂）分别抑制1-细胞期DNA的复制和合子基因组激活（ZGA）过程中组蛋白去乙酰化,探索MuERV-L基因表达的变化。结果表明：MuERV-L基因的转录在受精后9-11 h快速启动,并在2-细胞阶段出现瞬时高表达。抑制试验显示：1-细胞期DNA的复制受抑制后,MuERV-L基因的转录也被显著抑制（P〈0.05）;在TSA处理的4-细胞胚胎中,MuERV-L基因显著高表达（P〈0.01）,且其水平达到正常2-细胞的转录水平。提示：该基因可能对小鼠胚胎ZGA过程起重要作用。

The pattern of murine endogenous retrovirus-like gene （MuERV-L） mRNA expression was examined in the early preimplantation development of mice, using real-time RT-PCR technique. Effect of DNA replication and histone deacetylation on the zygotic genome activation （ZGA） of mouse in vitro fertilization （IVF） embryos was also investigated using 1-cell embryos treated with appidicolin and TSA, which inhibit DNA replication and the activities of histone deacetylase, respectively. The results indicated that transcription of the MuERV-L gene was rapidly activated from 9 to 11 h after IVF, then transiently increased during the 2-cell stage. In the inhibition experiment, MuERV-L gene transcription was downregulated significantly following DNA replication repressed in the 1-cell stage. Moreover, high expression of MuERV-L mRNA was observed in 4-cell embryos treated with TSA, and the transcriptional level of MuERV-L gene in 4-cell embryos achieved the same that in the normal 2-cell embryos. Conclusion： MuERV-L plays an important role in the ZGA of mouse embryos.