葛根素对低氧性肺动脉高压大鼠肺动脉压力及结构的影响

Intervention effects of puerarin on change of pulmonary artery pressure and structure in hypoxic pulmonary hepertension rats

目的观察大鼠在低氧状态下出现的肺动脉高压和肺小动脉结构的重构，以及葛根素对这两个病理变化的干预效应。方法雄性SD大鼠24只，随机分为对照组、低氧组和葛根素干预组。低氧组和葛根素干预组在常压低氧舱内建立肺动脉高压模型，葛根素干预组大鼠每天腹腔注射葛根素500mg／kg，测定各组平均肺动脉压，并取大鼠左肺沿肺门横断取材，石蜡包埋切片，进行苏木素伊红（HE）染色，观察肺小动脉形态学变化。结果①对照组和葛根素干预组大鼠平均肺动脉压均明显低于低氧组[（15．01±1．47）mmHg、（20．43±2．86）mmHg、（29．74±2．32）mmHg，P〈0．01]。②对照组大鼠肺小血管管壁较薄、管腔较大，低氧组大鼠肺小动脉普遍增厚，管腔狭窄，葛根素干预组的肺小动脉管壁及血管管腔狭窄程度较低氧组显著减轻。结论慢性缺氧可导致肺动脉壁增厚、管腔狭窄等血管重构病理改变；葛根素能在一定程度上抑制低氧肺动脉高压的形成和发展及肺血管结构的重构。

Objective To investigate the occurrence of pulmonary hepertension and the remodeling of pulmonary arteriolar structure in rats under hypoxia, and the interventional effects of puerarin on the two factors. Methods Twenty-four male SD rats were randomly allocated into three groups： control group, hypoxic group and puerarin interventional group （n = 8 in each group）. Rat models of pulmonary hypertension were established under discontinuous hypoxia in the normobaric hypoxic chamber for two weeks. Intraperitoneal injection of puerarin （500 mg/kg） was given to the rats in the puerarin interventional group every day immediately before hypoxia,and the others received injection of saline of the same volume. Mean pulmonary artery pressure was determined with pressure sensor. The left lungs of rats along the hilum of lung were sampled for, slicing embedded with olefin and stained with hematoxylin-eosin to observe the morphological changes of pulmonary small artery. Results ① The mean toulmonary artery pressure was obviously lower in control group and puerarin interventional group than in hypoxic group [（ 15.01 ± 1.47）, （20. 43±2.86）,（29.74±2.32） mm Hg, P 〈0.01]. ②The pulmonary small artery vascular wall was thinner and vascular cavity was bigger in control group. In hypoxic group, the pulmonary small artery was generally thickened,and the vascular cavity was constricted. In puerarin interventional group,the constricted severities of pulmonary small artery vascular wall and vascular cavity were significantly relieved than in hypoxic group. Conclusions ① Chronic hypoxia can result in the pathological changes of vascular reconstruction,such as thickening of pulmonary arterial wall and constriction of vascular cavity. ② Puerarin can inhibit the formation and development of hypoxic pulmonary hypertension and the remodeling of the pulmonary vascular structure to a certain extent.