维生素E对急性脊髓损伤治疗作用的实验研究

The Therapeutic effects of vitamin E on experimental spinal cord trauma

为了探讨维生素E对急性脊髓损伤治疗作用的实验研究，将40只雄性SD大鼠分治疗与对照组，用静压法造成脊髓损伤。治疗组伤后三天给予大剂量维生素E（口服200mg／d）o伤后一周内测定脊髓体感诱发电位（SSEP），伤后6周内测定下肢功能及病理检查。治疗组显示一周后SSEPN波幅值下降幅度小，保留部分约为伤前的75％。大白鼠下肢功能测定，伤后3周治疗组恢复较对照组好，脊髓病理检查也显示治疗组神经细胞较对照组好。实验结果表明，维生素E对急性脊髓损伤确有一定的治疗作用。

40 healthy SD rats weighting 300±20g were randomly divided into two groups: vitamin E group and control group. After laminectomy,the T spinal cord of rats was compressed with 4Og weght for 5 min. For 3 days after inury,treated rats received orally 200mg alpha tocopheral acetate (vitamin E)daily. Control rats were similarly injured butuntreated. All rats were blindly evaluated weekly for 6 weeks for their neurologic recovery(inclined plane test). SSEPwere mesured before and each day for one week after injury with DANTEIC SSEP recorder.The SSEP were measured in two groups in one week after injury,N amplitude of SSEP between V-E group and control group demonstrated respectively decline,but the level was lower in the V-E group than in the control group. Oneweek after injury,the V-E group showed higher recovery than the control group in the N amplitude of SSEP. The difference was markedly significant (P< 0.01 ). The observation of hindlimb function showed better recovery in V-E group.Treated rats had recovered 93 % of their preinjury function as compared with 79% for untreated controls. In histologicalexamination there were a less hemorrhagic spinal cord and more neurologic cells in V-E group. These results stronglysuggest that the alpha tocopheral exerts its effect on injured spinal cord tissue,at least in part,by its antioxidant and/orantipolytic activity.