L-精氨酸改性聚对苯二甲酸乙二醇酯膜的血液相容性

On Blood Compatibility of Polyethylene Terephthalate Films Modified with L-arginine

以戊二醛为交联剂,采用三步反应将L-精氨酸共价固定在聚对苯二甲酸乙二醇酯膜表面。用XPS和FT-IR检测了改性前后膜表面化学组成和结构的变化;通过接触角测定表征了膜表面化学组成对膜亲水性的影响;用BCA(bicinchoninic acid)法测定了膜表面吸附的蛋白量;借助扫描电镜观察了血小板在膜表面的粘附状况。结果显示:膜表面的水接触角由改性前的78.5°降低到改性后的43.7°;改性PET膜表面的蛋白吸附量比未改性PET的下降了25%;改性膜表面只有稀疏血小板粘附,且粘附的血小板呈圆形。

A three-step procedure was taken to covalently immobilize L-arginine to the surface of polyethylene terephthalate （PET） films using glutaraldehyde as a cross-linker. The composition and chemical structure of PET and its change with surface modification were examined by XPS and ATR-FT-IR, the change in hydrophilicity was judged by water contact angles measurement, the amount of protein adsorbed on the films was detected by bicinchoninic acid assay （BCA） method, and the morphologies of platelets adhered on various films were observed by scanning electron microscope （SEM）. The results show that L-Arg-modified films are more hydrophilic than PET with the contact angle decreasing from 78.3° for PET to 43.7° for PET-Arg; the amount of protein adsorption on L-Arg-modified films is decreased by about 25% compared with PET; and only a few platelets, round and not-aggregated, are adhered on L-Arg-modified films, which demonstrates that the blood compatibility of the L-Arg-modified films is significantly improved.