摘要
发绿拟青霉U－2原生质体形成和再生的较为适宜的条件为：YD培养48～52h，用0．15％流基乙醇预处理0．5h，以pH650．6MKCl作为渗透压稳定剂，用1％真菌治壁酶于28℃酶解4h；在合1％琼脂的再生培养基上再生。
The effects of media. cultivating time, enzyme and its concentratian, lytic temperature , osmotic pressure stabilizer and its concentration, and pretreatment to the protoplasm fromation and regeneration of paecforyces griseoriride U-2 strain and the effects of agar concentration in regenerate medium to the ratio of protoplasm regeneration of U-2 strain were studied in this paper. Results showed that the suitable conditions for protoplasm formation and regeneration of P.griseoviride U-2 were, cultivated in YD medium for 48-52 hours, treated with 1% lywallzyme at 28℃ in pH6. 5 0. 6mol/1 KCl for 4 hours and regenerated in RM containing 1% agar
出处
《生物技术》
CAS
CSCD
1997年第4期34-37,共4页
Biotechnology
关键词
真菌
灰绿拟青霉
原生质体
形成
再生
Paecilomyces griseoviride
protoplasm
formation and regeneration
