不同来源茶树油抑制LPS诱导TNF-α释放的体外实验

Inhibition effect on LPS-induced TNF-α release by homegrown and Australian Melaleuca alternifolia(Tea Tree)oil

目的比较国产和进口茶树油(tea tree oil,TTO)对脂多糖(lipopolysaccharide,LPS)诱导炎症的抑制作用。方法四唑盐(MTT)比色实验筛选无细胞毒性作用的TTO溶液浓度;各浓度TTO水溶液按量效和时效关系预处理细胞后,测定LPS激活RAW264.7细胞分泌肿瘤坏死因子-α(tumor necrotic factor-alpha,TNF-α)的浓度,以观察国产和进口TTO水溶液对LPS诱导炎症的抑制效果。结果浓度低于0.001%的国产和进口TTO溶液对细胞无毒性作用;0.000125%～0.001%进口TTO和0.00025%～0.001%国产TTO各浓度组均能非常显著地抑制LPS诱导细胞释放TNF-α;0.00025%进口和国产TTO分别在3h和6h内对细胞有显著的保护作用。结论国产和进口TTO对LPS诱导的炎症均有显著抑制效果。

Objective To compare the inhibition of the homegrown and Australian tea tree oil （TTO） solution on TNF-α release from RAW264.7 cells induced by LPS. Methods MTT assay was used to measure the effects of TTO on viability of RAW264.7 in vitro. RAW264.7 cells were pretreated with two kinds of TTO solution in the dose- and time-dependent manners, then stimulated by LPS. TNF-α in the supernate was tested by ELISA. Results In vitro, two kinds of TTO （ 〈 0. 001% ） showed no cytotoxicity to RAW264.7 cells. Australian TTO （0. 000 125 % - 0.001% ） and homegrown TTO （ 0. 000 25 % - 0.001% ） significantly suppressed LPS-induced TNF-α release, respectively. 0. 000 25% Australian TTO pretreating for 3 h and homegrown TTO pretreating for 6 h showed significant protections against the stimulations of LPS, respectively. Conclusion In vitro, two kinds of TTO have the obvious inhibitory action, and there is no significant difference between Australian and homegrown TTO.