戊四氮癫痫模型失神发作期脑电图及意识状态的实验研究

EEG alterations and conscious state of epileptic rats during absence seizure induced by pentylenetetrazol

目的探讨戊四氮失神发作癫痫模型在发作期是否伴有特异性的痫样放电和意识障碍,为判断该模型是否为可靠的失神发作癫痫模型提供确切的实验依据。方法①记录10只戊四氮模型大鼠癫痫发作期的脑电图,以证实是否伴有特异性的脑电图痫样放电。②利用双向穿梭箱,以主动回避反应(active avoidance response,AAR)、被动回避反应(passive escape response,PER)及逃逸失败的阳性率为观察指标,判断大鼠痫性发作期的意识状况。结果①戊四氮注射大鼠痫性发作期均伴有双侧同步的棘波发放。②癫痫发作中,戊四氮注射大鼠主动回避反应潜伏期(active avoidanceresponse latency,AARL)明显延长,AAR阳性率下降85%,PER阳性率增加56%,逃逸失败阳性率增加29%。结论戊四氮注射大鼠可致特异性的痫性放电,同时并存意识障碍。

Objective To evaluate whether the pentylenetetrazol （PTZ） model is an appropriate animal model of absence seizure. Methods A series of tests including EEG, active avoidance response （AAR） and passive escape response （PER） in a two-way shuttle box were finished during seizure induced by PTZ （35 mg/kg, ip） in 40 Wistar epileptic rats. Results Epileptiform activity was characterized by bilaterally spike wave discharges synchronously elicited during seizure in PTZ models. During absence seizure induced by PTZ, the latency of active avoidance response was significantly prolonged. AAR positive rate was decreased by 85%. PER positive rate was increased by 56%, and escape failure positive rate was increased by 29%. Conclusion There is epileptiform discharge accompanying transient conscious disturbance during absence seizure induced by pentylenetetrazol.