CdTe量子点标记铁蛋白的光谱研究

Study on Spectra of CdTe Quantum Dots Labeling Ferritin

采用水相合成的CdTe量子点标记铁蛋白,利用荧光和飞秒技术系统地考察了标记前后体系的发射光谱和发光寿命的变化。与单独CdTe量子点相比,标记了铁蛋白的量子点发射光谱半峰宽不变,发光强度随着铁蛋白的量增加而降低,并且发光寿命没有改变。通过改变体系的pH值、铁蛋白量等各种条件验证了CdTe-Ferritin溶液发射光谱和发光寿命的变化确实是由于铁蛋白和CdTe量子点之间的结合反应引起的。

The ferritin can be labeled with CdTe quantum dots synthesized in aqueous solution. The changes of emission spectra and lifetime of CdTe quantum dots before and after labeling ferritin were studied by UV and fluorescence spectrophotometries. The emission lifetime of CdTe quantum dots bound to ferritin remained unchanged, but the PL intensity was decreased with increasing amount of ferritin. The fact that the changes of emission intensity and luminescence lifetime are due to the binding of CdTe and ferritin is further confirmed through experiments by changing pH values and ferritin amount of test solution.