利用纤维素酶提取笃斯越桔花青素的研究

Study on Extraction of Anthocyanins in Vaccinium uliginosum L. with Cellulase

本实验研究纤维素酶法提取笃斯越桔中的花青素的工艺条件,重点考察了料液比、酶用量、温度、pH及酶解时间等因素对花青素提取率的影响,通过L16(44)正交试验优化出酶法提取的长白山野生笃斯越桔果实中花青素的最佳工艺参数为:料液比1:50,pH为1.5,加酶量2%,温度60℃,酶解时间1h。在此条件下,花青素提取率为6.105%。

Cellulase was used to extract anthocyanins in wild Vaccinium uliginosum L. from Changpbai Mountain area, and then the ratio of solid to liquid, the amount of Cellulase, the temperature, the pH and the time of enzymolysis were investigated emphatically. Through single factors and L16（4^4） orthogonal design on the extraction process, the optimal parameters were obtained as follows： the ratio of solid to liquid 1：50, pH 1.5, the amount of Cellulase 2%, temperature 60 ℃ and time of enzymolysis 1 h. Based on the above conditions, the extraction rate was 6.105%.