引种新资源——印度人参的功能成分定性及睡茄内酯的分离鉴定

New Resource Introduced ——Study on Functional Composition of Withania Somnifera(L.) Dunal

目的:对引种新资源植物印度人参(Withania somnifera(L.)Dunal)主要功能成分定性及睡茄内酯的分离鉴定。方法:借助系统预试验初步判断引种资源印度人参可能含有的功能成分类型。采用有机溶剂提取、萃取、柱层析、薄层层析和溶剂结晶等手段相结合,得到一个睡茄内酯单体化合物;采用熔点、IR、UV、MS和NMR等方法进行鉴定。结果:定性分析提示其含有机酸、酚类及黄酮类、强心苷、甾体皂甙、生物碱、内酯、香豆素及苷类化合物等。鉴定1个单体化合物为5,20-二-OH-6,7-环氧-1-羰基-睡茄-2,24-烯。结论:引种新资源印度人参所含主要功能成分与国外报道一致。

Objective： Qualitative analye of the main functional compositions and seperation and identification of withanolides of Withania somnifera（L.）Dunal（Ashwagandha）cultivated in Chongqing. Methods： With the help of system pro-experiment to preliminarily estimate the functional compositions in Ashwagandha introduced from abroad. A monocase compound was isolated by organic solvent extraction with VLC, TLC as well as recrystallization and then was identified by melting point UV,/R, NMR and MS. Results： The Ashwagandha introduced from abroad may contain organic acids, phenols and flavonoids, cardiac glycosides, steroidal saponins, alkaloids, olides and coumarin as well as glycosides and others. The monocase compound is 5,20- two-hydroxy-6,7-epoxy-l-carbonyl-withania-2,24-alkene. Conclusion： Ashwagandha Introduced from aborad contains the same functional compositions as those reported from overseas.