固相萃取-气相色谱法测定农产品中嘧霉胺的残留量

Determination of Pyrimethanil Residue in Agro-Products by SPE-GC

建立了青菜、大米、草莓、葡萄和西红柿等样品嘧霉胺残留量的SPE-GC测定方法。样品经乙腈提取,液-液分配和SPE净化,用气相色谱分法(NPD)检测器,外标法定量。实验结果:添加浓度在0.1～2.5mg/kg之间,测得其回收率为83.44%～108.82%之间,方法的检出限为0.05mg/L,同一样品中5次测定嘧霉胺残留量的相对标准偏差小于5%。

Method for the determination of pyrimethanil residue in pakchoi, rice, strawberry, grape, and tomato by means of SPE-GC was developed, respectively. The sample of pyrimethanil in agro-products was extracted with acetonitrile, and cleaned up by liquid-liquid partition with Silica gel SPE. The final purified extract was analyzed by GC/NPD with the external standard method. The recoveries of various amounts of pyrimethanil spiked （0.1 ～2.5mg/kg） samples of selected agro-products are in the range of 83.44% ～ 108.82%..The detection limit is 0.05mg/L and the RSD（n=5） ranges from of 0.93% to 4.82% .The results showed that the method meets the standard of the residue analysis.