酸性染料比色法测定两面针药材总生物碱的含量

Determination of Total Alkaloid in Zanthoxylum nitidum(Roxb.)DC. by Bromophenol Blue Colorimetry

以氯化两面针碱为标样,采用溴麝香草酚蓝为酸性染料,在总生物碱与其形成稳定络合物时间范围内,利用比色法测定两面针药材中总生物碱的含量,测定波长为393nm。两面针总生物碱检测浓度在2.56～10.26μg/ml范围内线性关系良好(r=0.9997),平均回收率为101.00%(RSD=2.46%,n=6)。该法测定两面针总生物碱含量简便快捷,准确易行。

The content of total alkaloid in Zanthoxylum nitidum（Roxb.） was determined by bromophenol blue colorimetry, and the UV-visible spectrophometry was adopted to determine the total alkaloid with the wavelength at 393 nm. The total alkaloid is in linear relationship within the concentration range of 2.56～ 10.26 μ g/ml（r=-0.9997）, the average recovery rate is 101.00% （RSD=2.46%, n=6）. Bromophenol blue colorimetry is simple, quick and accurate in the determination of total alkaloid in ZanthoxyLum nitidum （Roxb.）DC.