葫芦巴多糖酸解和酶解产物抗氧化及抗菌活性的比较研究

Antioxidant and Antimicrobial Activity of Acidolysis and Enzymolysis Products of Fenugreek Polysaccharides

本实验在成功研究葫芦巴多糖的酸解和酶解的基础上比较研究了葫芦巴多糖不同分子量的酸解和酶解产物的抗氧化活性以及对几种植物病原菌的抑菌作用。结果表明:酸解和酶解产物均具有一定的体外清除超氧阴离子(O2·)和羟自由基(·OH)的能力。接种淋巴癌细胞(S180)的小鼠实验结果显示,酸解和酶解产物均能抑制小鼠肝、肾等内脏器官超氧化物歧化酶(SOD)活性下降,减少脏器中膜脂过氧化产物丙二醛(MDA)生成量。两种水解产物对4种植物病原菌即葡萄灰酶、蚕豆褐班、棉红腐、茄子菌核均具有较为明显的抑制作用,并呈现明显的量效关系。比较而言,葫芦巴多糖的酶解产物的抗氧化活性以及抗菌作用强于酸解产物。

Antioxidant activity and inhibitory effects on plant pathogenic fungi of the acidolysis and enzymolysis products with different molecular weights of fenugreek polysaccharide were studied in this paper. Results showed that： acidolysis and enzymolysis products have effects on cleating hydroxyl radical （· OH ）and superoxide anion（O2^-·）. They inhibit the decline of superoxide dismutase （SOD） activity of liver and nephros of S 180 mice, and reduced the production of MDA. These two kinds of products both have obvious inhibitory effects on 4 plant pathogenic fungi： Botrytis cinerea, F.moniliforme, Ascochyta fabae speg and Eggplant Verticillicum wilt. And their dose-effect relationship is obvious. Enzymolysis products have more obvious effects on antioxidant and antimicrobial activity than acidolysis products.