泌尿系细菌L型感染及其对常用抗生素的耐药性检测

Detection of bacterial of L-forms and its antibiotic resistance in patients with urinary infection

目的探讨泌尿系细菌L型感染情况及其对常用抗生素的耐药性,为临床诊治提供科学依据。方法用改良Kagan培养基对临床疑为泌尿系感染者的尿液分离培养,并对分离获得的细菌L型进行药物敏感试验。结果泌尿系L型细菌感染率为42.17%(35/83),其中大肠埃希氏菌L型占37.14%(13/35)。分离出的细菌L型对丁胺卡那霉素、庆大霉素、诺氟沙星、环丙沙星、青霉素G等耐药性较高。结论临床对疑为泌尿系感染者应同时检测细菌及其L型,并根据药敏试验结果联合用药,以杀菌并阻止其变为L型。

Objective To study bacterial of L-forms and its antibiotic resistance in patients with urinary infection and to provide scientific evidences for clinical diagnosis and treatment. Methods 83 urine samples were cultivated with ordinary culture media and L-form bacteria culture media, and drug sensitivity tests were conducted. Results The infection rate of bacterial L-forms was 42.17% （35/83） in patients with urinary infection. Of these bacterial L-forms, 37.14% （13/35） was Escherichia coli. Amikacin, gentamicin, norfloxacin, ciprofloxacin, and penicillin G represented high resistance rate to bacterial L-forms. Conclusion Bacteria and its L-forms should be detected at the same time for patients with urinary infection, and the two forms should be considered together in clinical diagnosis and treatment.