人工磷脂双分子层模型的原子力显微镜研究

To Study the Height and Topography of Lipid Bilayer by AFM

目的:通过原子力显微镜从纳米水平成像人工磷脂双分子层的结构细节。方法:采用囊泡融合法形成了单组份和多组份人工磷脂双分子层模型,通过原子力显微镜进行观察。结果:通过原子力显微镜在纳米水平观察到了人工磷脂双分子层和其流动性;同时进行磷脂双分子层高度测量。结论:原子力显微镜是进行人工磷脂双分子层模型研究的良好方法。为细胞膜的进一步研究打下基础。

Objective： To image the structure of the lipid bilayer at the nanometer level with AFM. Methods： AFM images the lipid bilayer that was formed by the vesicle fusion method. Results： The fluidity of the lipid bilayer can be investigated with AFM in fluid. The height and topography of the lipid bilayer can be calculated and observed by AFM. Conclusion： AFM is a good tool to image the lipid bilayer.