口服糖皮质激素大鼠皮肤组织形态学变化

Morphological changes in rat skin following intragastric administration of glucocorticoid

目的:观察口服糖皮质激素大鼠皮肤形态学改变,为建立新的皮肤衰老动物模型提供形态学上的依据。方法:实验于2006-07/2007-02在广东医学院药理教研室组织药理实验室完成。①实验对象:20只SD雄性SPF级大鼠随机分为两组,糖皮质激素组和空白对照组,每组10只。②实验方法:糖皮质激素组每天口服灌胃糖皮质激素3.5mg/(kg·d),空白对照组每天口服灌胃同等剂量生理盐水,喂养100d后麻醉下处死大鼠,取背部正中1cm×1cm大小皮肤组织检测相关指标。③实验评估:常规苏木精-伊红染色、VanGieson染色法、Weigert-间苯二酚-碱性品红染色法观察皮肤组织形态改变,并用计算机图像分析系统定量分析表皮厚度和弹力纤维总面积。结果:纳入结果分析16只,其中空白对照组8只,糖皮质激素组8只。糖皮质激素组大鼠皮肤表皮变薄,弹力纤维面积减少,胶原纤维多断裂,排列疏松,糖皮质激素组大鼠表皮厚度(33.8±3.1)μm,弹力纤维总面积(3557.9±373.1)μm2,均小于对照组大鼠表皮厚度(63.7±7.4)μm,弹力纤维总面积(5049.0±497.5)μm2。结论:糖皮质激素3.5mg/(kg·d)口服灌胃100d可导致大鼠皮肤衰老样改变,与人类皮肤衰老有相似形态学表现。

AIM： To observe the morphological changes in rats with glucocorticoid-induced skin aging, so as to provide morphological evidence for establishing new animal models of skin aging. METHODS： The experiment was conducted in the Laboratory of Department of Pharmacology, Guangdong Medical College from July 2006 to February 2007. ① Twenty male SD rats of SPF grade were randomly divided into glucocorticoid group and control group with 10 rats in each group. ②The rats in glucocorticoid group were intragastrically administrated with glucocorticoid 3.5 mg/kg daily, wile the control group with normal saline. The rats were sacrificed after 100 days under anesthesia. Then the skin samples of 1 crnxl cm from the middle back was harvested for morphological measurement. ③The cutaneous histopathology was observed with HE staining, Van Gieson staining, and Weigert-rosorcin-aniline rod staining, and the epidermal thickness and area of elastic fibers were determined quantitatively with imaging analysis system. RESULTS： Totally 16 rats were involved in the result analysis including 8 in control group and 8 in glucocorticoid group. In glucocorticoid group, epidermal thickness and content of elastic fibers were decreased and collagen fibers were rarefaction, most of which were ruptured. Both epidermal thickness （33.8±3.1） μm and area of elastic fibers （3 557.9±373.1）μm2 in glucocorticoid group were lower than control group （63.7±7.4）μm and （5 049.0±497.5）μm^2. CONCLUSION： The intragastric administration of glucocorticoid （3.5 mg/kg daily） for 100 days induces skin aging in rats, and the changes of morphology are similar to those of human being.