期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

法尼酯X受体对脂肪酸合酶表达的影响 被引量：2

Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells
下载PDF
导出
摘要 目的探讨法尼酯X受体(farnesoid X receptor,FXR)对脂肪酸合酶(fatty acid synthetase,FAS)表达的影响。方法用FXR激动剂CDCA作用人肝癌细胞株(HepG2),应用RT-PCR和Western blotting分别从mRNA水平和蛋白质水平检测FAS的表达情况。结果用不同浓度的CDCA(25、50、75μmol/L)分别作用于HepG2细胞,6、12、24、48h后,FASmRNA和蛋白质水平呈时间和剂量依赖性下调。结论FXR激动剂可抑制脂肪酸合酶的表达。 Objective To investigate the effect of the farnesoid X receptor （FXR） on fatty acid synthetase expression. Methods Human hepatoblastoma HepG2 cells were treated with a FXR＇s agonist, chenodeoxycholic acid （CDCA） at 75 ttmol/L for 6, 12, 24 and 48 h respectively, or at 25, 50 and 75 μmol/L for48 h. Expressions of FAS mRNA and protein were determined by RT-PCR and Western blot analysis respectively. Results RT-PCR and Western blot analysis indicated that the mRNA and protein were expressed in a time and dose-dependent manner in the HepG2 cells treatmented by CDCA. Conclusion FXR agonist decreases the expression of fatty acid synthetase.
作者 申丽丽 彭家和 刘红 彭彦孟 甘淋 鲁立 张迁 江渝
机构地区 第三军医大学基础医学部生物化学与分子生物学教研室 第三军医大学新桥医院血液内科 第三军医大学学员旅
出处 《第三军医大学学报》 CAS CSCD 北大核心 2007年第22期2142-2144,共3页 Journal of Third Military Medical University
基金 第三军医大学留学回国人员启动基金(2006) 重庆市自然科学基金计划项目(2007BB5050)~~
关键词 法尼酯X受体 脂肪酸合酶 动脉粥样硬化 甘油三酯 胆汁酸 farnesoid X receptor fatty acid synthetase atherosclerosis
分类号 Q591.5 [生物学—生物化学] R394 [医药卫生—医学遗传学]
  • 相关文献

参考文献10

  • 1Nakamura T, Kugiyama K. Triglycerides and remnant particles as risk factors for coronary artery disease [ J]. Curr Atheroscler Rep, 2006, 8 (2): 107-110.
  • 2Sinai C J, Tohkin M, Miyata M, et al. Targeted disruption of the nuclear receptor FXR/BAR impairs bile acid and lipid homeostasis [ J ]. Cell, 2000, 102(6) : 731 -744.
  • 3Makishima M. Nuclear receptors as targets for drug development : regulation of cholesterol and bile acid metabolism by nuclear receptors[ J]. J Pharmacol Sci, 2005, 97(2) : 177 -183.
  • 4Rizzo G, Renga B, Mencarelli A, et al. Role of FXR in regulating bile acid homeostasis and relevance for human diseases[ J]. Curr Drug Targets Immune Endocr Metabol Disord, 2005, 5 (3) :289 - 303.
  • 5Jayakumar A, Tai M H, Huang W Y, et al. Human fatty acid synthase: properties and molecular cloning[ J]. Proc Natl Acad Sci U S A, 1995, 92 (19) : 8695-8699.
  • 6Sit'vent A, Verhoeven A J, Jansen H, et al. Farnesoid X receptor re presses hepatic lipase gene expression [ J ]. J Lipid Res, 2004, 45 (11): 2110-2115.
  • 7Angelin B, Einarsson K, Hellstrom K, et al. Effects of eholestyramine and chenodeoxycholic acid on the metabolism of endogenous triglycefide in hyperlipoproteinemia[J]. J Lipid Res, 1978, 19(8): 1017- 1024.
  • 8Kast H R, Nguyen C M, Sinai C J, et al. Farnesoid X-activated receptor induces apolipoprotein C- Ⅱ transcription : a molecular mechanism linking plasma triglyceride levels to bile acids [ J ]. Mol Endocrinol, 2001, 15(10) : 1720-1728.
  • 9Anisfeld A M, Kast-Woelbern H R, Meyer M E, et al. Syndecan-1 expression is regulated in an isoform-specific manner by the farnesoid-X receptor[J]. J Biol Chem, 2003, 278 (22) : 20420 -20428.
  • 10Matsukuma K E, Bennett M K, Huang J, et al. Coordinated control of bile acids and lipogenesis through FXR-dependent regulation of fatty acid synthase[J]. J Lipid Res, 2006, 47(12) : 2754-2761.

同被引文献15

  • 1Huber R M, Murphy K, Miao B, et al. Generation of multiple fame-sold-X-receptor isoforms through the use of ahemative promoters [ J ]. Gene, 2002, 290(1/2) : 35 -43.
  • 2Willson T M, Jones S A, Moore J T, et al. Chemical genomics: functional analysis of orphan nuclear receptors in the regulation of bile acid metabolism[J]. MedResRev, 2001, 21(6):513-522.
  • 3Cariou B, van-Harmelen K, Duran-Sandoval D, et al. The farnesoid X receptor modulates adiposity and peripheral insulin sensitivity in mice [J]. J Biol Chem,2006, 281(16): 11039-11049.
  • 4Watanabe M, Houten S M, Wang L, et al. Bile acids lower triglycefide levels via a pathway involving FXR, SHP, and SREBP-lc[J]. J Clin Invest , 2004, 113(10) : 1408 - 1418.
  • 5Wang Y D, Chen W D, Huang W. FXR, a target for different diseases [ J ]. Histol Histopathol, 2008, 23 (5) : 621 - 627.
  • 6Pineda-Torra 1, Claudel T, Duval C, et al. Bile acids induce the ex- pression of the human peroxisome proliferator-activatcd receptor alpha gene via activation of the farnesoid X receptor [J].Mol Endocrinol, 2003, 17(2) : 259 -272.
  • 7Zhang Y, Castellani L W, Sinal C J, et al. Peroxisome proliferator- activated receptor-gamma coactivator I alpha ( PGC-I alpha ) regulates triglyceride metabolism by activation of the nuclear receptor FXR [ J ]. Genes Deny, 2004, 18(2): 157-169.
  • 8Storch J, Thumser A E. The fatty acid transport function of fatty acid- binding proteins[J]. Biochim Biophys Acta, 2000, 1486 (1) : 28 -44.
  • 9NewbeiTy E P, Xie Y, Kennedy S, et al. Decreased hepatic triglyceride accumulation and altered fatty acid uptake in mice with deletion of the liver fatty acid-binding protein gene [ J ]. J Biol Chem, 2003, 278(51 ) : 51664 -51672.
  • 10Newberry E P, Xie Y, Kennedy S M, et al . Protectiun against Western diet-induced obesity and hepatic steatosis in liver fatty acidbinding protein knockout mice[J].Hepatology, 2006, 44 (5) : 1191 - 1205.

引证文献2

二级引证文献12

第三军医大学学报
2007年 第22期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部