一个绿竹MADS-box基因的克隆与序列分析

Cloning and Sequencing Analysis of a Flowering-related MA DS-box Gene in Bambusa oldhamii L.

MADS-box基因编码的转录因子在植物花器官发育过程中起着重要作用。采用RT-PCR技术,从绿竹(Bambusa oldhamii L.)中分离到一个MADS-box基因,命名为BOMADS1(GenBank登记号:EF517293)。BOMADS1编码区cDNA全长723 bp;编码区共编码240个氨基酸,具有典型的植物MADS-box基因结构,其编码肽链包含了MADS区、K区、I区和C末端。序列分析结果表明,BOMADS1编码的蛋白与其它植物的MADS-box蛋白有着较高的一致性,其中与石刁柏(Asparagus officinalis L.)的AOM3高达87%。系统进化树分析表明BOMADS1基因属于E类功能基因。

Floral homeotic MADS-box genes encoding transcription factors play important roles in determining floral organ identities. The flowering-related MADS-box gene, named as BOMADS1 （GenBank accession number： EF517293）, was cloned from the first strand of Bambusa oldhamii L. cDNA through RT-PCR using primers designed according to the MADS-box gene family conserved region. The fragment was 723 bp length and contained an open reading frame coding a polypeptide of 240 amino acids which had a typical MADS-box gene structure including MADS-box domain, K domain, I region and C terminus. Homology analysis indicated that the deduced BOMADS1 protein was highly homologous to other MADS-box proteins from different species, especially with AOM3 from Asparagus officinalis up to 87%. Phylogenetic tree analysis also indicated that BOMA DS1 might belong to E function genes.