半导体量子点对原代培养大鼠牙乳头细胞生物相容性的研究

Study on the biocompatibility of semiconductor quantum dots to the rat dental papillae cells (RDPC) cultured in vitro

目的:研究半导体量子点(QDs)对原代培养的大鼠牙乳头细胞(Rat dental papillae cells,RDPCs)的生物相容性。方法:①选择刚出生3天的新生SD大鼠,取出磨牙牙胚,分离牙乳头,用酶消化法培养SD大鼠牙乳头细胞,用波形丝蛋白和细胞角蛋白免疫化学染色鉴定细胞来源,观察细胞的生长规律;②把605QDs、525QDs及两种量子点等浓度的混合物按3种不同浓度与RDPCs共同培养,观察QDs对RDPCs生长和形态的影响;③用MTT法检测不同浓度的605QDs、525QDs及两种量子点等浓度的混合物对RDPCs的毒性作用结果:①:体外培养的RDPCs生长良好,波形丝蛋白染色阳性而细胞角蛋白染色阴性。②不同浓度的605QDs、525QDs及两种量子点的等浓度混合物对体外培养的RDPCs没有毒性作用,对其生长和形态也没有影响。结论:605QDs、525QDs及两种量子点等浓度的混合物在一定的浓度范围内时RPDCs具有良好的生物相容性,为605QDs、525QDs在活体生理条件下用于活细胞内的多通道信号研究提供了科学依据。

Objective：To study the biocompatibility of semiconductor quantum dots to the rat dental papillae cells （RDPCs） cultured in vitro. Methods：①RDPCs isolated and cultured with enzyme digestion. Wave form fibroin and cytokeratin were used to identify the cells resource and the growth rules of cells was observed. ②To study the effect of QDs to the RDPCs,The RDPCs were cultured with the 605QDs,525QDs and those mixture in different density.③With the MTr,to study the cytotoxicity of QDs to the RDPCs,The RDPCs were cultured with the 605QDs,525QDs and those mixture in different density. Results：① RDPCs cultured in vitro were well growing. Wave form fibroin staining was positive and cytokeratin staining was negative in RDPCs. ②The 605QDs,525QDs and those mixture in different density show no negative effect and cytotoxicity on the growth of the RDPCs. Conclusion：The 605QDs,525QDs in a range of density have well biocompatibility to the live cells,which supplied scientific evidence for the research of muhichannel signal in living cells in vivo physiological condition