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CTLA-4抗体诱导原因不明复发性流产患者Th1／Th2转换的体外研究 被引量：4

In vitro study on Th1/Th2 shift modulated by anti-CTLA4 antibody in unexplained recurrent spontaneous abortion
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摘要 目的:观察CTLA-4抗体在诱导原因不明复发性流产患者Th1/Th2转换中的作用。方法:用人绒毛膜癌细胞株JEG-3制备的滋养细胞抗原刺激30例复发性流产患者外周血单核细胞,实验组加CTLA-4抗体(10μg/ml组和1μg/ml组),对照组加IgG(10μg/ml组和1μg/ml组)培养,分别取24h、72h上清液,ELISA法测其IL-2、IFN-γ和IL-4的水平。结果:①与同浓度对照组相比,实验组产生的IL-4均显著降低(P<0.01),而产生的IFN-γ、IL-2均明显升高(P<0.05)。②与CTLA-4抗体1μ/ml组相比,CTLA-4抗体10μg/ml组产生的IL-4稍低,而IFN-γ和IL-2稍高,但无统计学差异(P>0.05),结论:CTLA-4抗体可以上调Th1型细胞因子,下调Th2型细胞因子,提示CTLA-4抗体体外阻断CTLA-4可诱导原因不明复发性流产的Th1/Th2失衡更向Th1极化,不利于妊娠。 Objective：To investigate the effects of anti-CTLA4 antibody on Th1/Th2 shift in unexplained recurrent spontaneous abortion. Methods：The levels of IL-2,IFN-γ and IL-4 were assessed by ELISA in the supernatant of cultured maternal peripheral blood mononuclear cells（PBMC） from 30 patients of Unexplained RSA. Cytokine synthesis was induced by activation with antigens from a choriocarcinoma cell line of JEG-3,with anti-CTLA4 antibody or IgG. Results：We detected significantly greater levels of the Thl cytokines IL-2,IFN-γ（P〈0.05）and lower levels of the Th2 cytokines IL-4（P〈0.01 ）in anti-CTLA4 antibody groups,compared with control group of the same concentration. Between the trial groups of different concentration, there is no significance（P〉0.05）. Conclusion：Anti-CTLA4 antibody can up-regulate Thl-type cytokines and down-regulate Th2-type cytokines, which indicates that in URSA anti-CTLA4 antibody could swith T cell responses to Th1,and is harmful to URSA.
作者 刘西茹 李尚为 汪燕 樊伟
机构地区 重庆医科大学附属第一医院辅助生殖中心 四川大学华西第二医院生殖中心
出处 《重庆医科大学学报》 CAS CSCD 2007年第12期1265-1267,1307,共4页 Journal of Chongqing Medical University
关键词 流产 复发性 TH1细胞 TH2细胞 CTLA-4 Abortion, Recurrent Th 1 cells Th2 cells Cytotoxic T lymphocyte associated antigen4
分类号 R714.21 [医药卫生—妇产科学]
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参考文献12

  • 1Regan L and Rai R. Epidemiology and the medical cause of miscarriage[J]. Bailliere's Clin. Obstet Gynaeeol,2000; 14: 839-854.
  • 2邱丽华,林其德,洪燕,李东至,丁传伟.原因不明习惯性流产患者主动免疫治疗前后T辅助细胞1、2型细胞因子的变化[J].中华妇产科杂志,2001,36(7):408-410. 被引量：55
  • 3Ubaldi V,Gatta L,Paee L,et al. CTLA-4 engagement inhibits Th2 but not Thl cell polarization[J]. Clin Dev Immunol,2003;10(1): 13-17.
  • 4Zhu Ji, Zou Liping,Zhu Shunwei,et al. Cytotoxic T lymphocyte associated antigen 4 (CTLA-4) blockade enhances incidence and severity of experimental autoimmuneneuritis in resistant miee[J]. J Neuro Irnmunol,2001 ; 115(5) : 111-113.
  • 5Hill JA,Polgar K,Harlow BL,et al. Evidence of embryo-and trophoblast-toxic cellular immune response (s) in women with recurrent spontaneous abortion[J]. Am J Obstet Gynecol,1992;166(4):1044-1052.
  • 6Mellor AL,Munn DH. Immunology at the maternal-fetal interface:lessons for T cell tolerance and suppression[J]. Annu Rev Immunol, 2000;18:367-391.
  • 7Wang X,Ma Z,Hong Y,et al. Expression of CD28 and cytotoxic T lymphocyte antigen 4 at the maternal-fetal interface in women with unexplained pregnancy los[J]. Int J Gynaecol Obstet,2006; 93(2) : 123-129.
  • 8汪希鹏,林其德,马政文,洪艳,赵爱民,狄文,陆佩华.原因不明复发性流产患者细胞毒性T淋巴细胞抗原4基因第一外显子49位点A/G基因多态性研究[J].中华妇产科杂志,2006,41(3):155-158. 被引量：5
  • 9Collins M, Carreno BM. The B7 family of ligands and its receptors:new patheways for costimulation and inhibition of immune responses[J]. Ann Rev Immunol,2002;20:29.
  • 10Kato T,Nariuchi H. Polarization of naive CD4^+ T cells toward the Thl subset by CTLA-4 costimulation[J]. J Immunol,2000;164(7): 3554-3562.

二级参考文献26

  • 1林其德,周涵春.原因不明性习惯性流产的免疫学诊断与治疗[J].中华妇产科杂志,1993,28(3):143-146. 被引量：41
  • 2林其德,赵爱民,周涵春,汤希伟,严隽鸿,吴宇芬.原发性习惯性流产的主动免疫治疗[J].中华妇产科杂志,1996,31(6):351-352. 被引量：41
  • 3Mellor AL,Munn DH.Immunology at the maternal-fetal interface:lessons for T cell tolerance and suppression.Annu Rev Immunol,2000,367-391.
  • 4Frauwirth KA,Thompson CB.Activation and inhibition of lymphocytes by costimulation.J Clin Invest,2002,109:295-299.
  • 5Ueda H,Howson JM,Esposito L,et al.Association of the T-cell regulatory gene CTLA4 with susceptibility to autoimmune disease.Nature,2003,423:506-511.
  • 6Lee CS,Lee YJ,Liu HF,et al.Association of CTLA4 gene A-G polymorphism with rheumatoid arthritis in Chinese.Clin Rheumatol,2003,22:221-224.
  • 7Harper K,Balzano C,Rouvier E,et al.CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequency,message expression,gene structure,and chromosomal location.J Immunol,1991,147:1037-1044.
  • 8Kinjo Y,Takasu N,Komiya I,et al.Remission of Graves′ hyperthyroidism and A/G polymorphism at position 49 in exon 1 of cytotoxic T lymphocyte-associated molecule-4 gene.J Clin Endocrinol Metab,2002,87:2593-2596.
  • 9Fournel S,Aguerre-Girr M,Huc X,et al.Cutting edge:soluble HLA-G1 triggers CD95/CD95 ligand-mediated apoptosis in activated CD8+ cells by interacting with CD8.J Immunol,2000,164:6100-6104.
  • 10Makrigiannakis A,Zoumakis E,Kalantaridou S,et al.Corticotropin-releasing hormone promotes blastocyst implantation and early maternal tolerance.Nat Immunol,2001,2:1018-1024.

共引文献57

同被引文献29

  • 1许希中,余进进,陈小平,翟蓉萍,吴海燕.妊娠相关蛋白-A及其它妊娠相关激素对早期不良妊娠结局的预测价值探讨[J].现代保健（医学创新研究）,2006(12):1-3. 被引量：2
  • 2胡桂英.早产.见:乐杰.妇产科学[M].6版.北京:人民卫生出版社,2005:92-94.
  • 3Raghupathy R.Cytokine production by maternal lymphoeytes during normal human pregnancy and in unexplained recurrent spontaneous abortion[J].Hum Reprod,2000 (3):713 -718.
  • 4Paradisi R,Maldini-Casadei M,Boni P,et al.T-helper 2-cytokine levels in women with threatened abortion[J].Eur J Obstet Gynecol Reprod Biol,2004 (2):239 -240.
  • 5Steven O.Maternal periodontal infections,prematurity,and growth restriction[J].Clin Obstet' Gyneeol,2004 (4):808 -821.
  • 6Maymon E,Romero R,Pacora P,et al.A role for the 72 kDa gelatinase (MMP-2) and its inhibitor (TIMP-2) in human parturition,premature rupture of membranes and intraamniotic infection[J].J Perina Med,2001 (4):308 -316.
  • 7Bulmer JN, Lash GE. Human uterine natural killer cell: a reappraisal[ J ]. Mol hnmunol, 2005,42 (10) :511 - 521.
  • 8Koopman LA, Kopcow HD, Rybalov B, et al. Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential[ J]. J Exp Med, 2003,198(8) :1201 - 1212.
  • 9Paradisi R, Maldini-Casadei M, Boni P, et al. T-helper 2-cytokine levels in women with threatened abortion [ J]. Eur J Obstet Gynecol Reprod Biol, 2004,114 ( 2 ) :239 - 240.
  • 10Steven O. Maternal periodontal infections, prematurity, and growth restriction[ J].Clin Obstet Gyneeol, 2004,47 (4) :808 - 821.

引证文献4

二级引证文献19

重庆医科大学学报
2007年 第12期

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