摘要
Cancer gene discovery continues to drive current cancer research with the promise of identifying new diagnostic markers and therapeutic targets by elucidating novel genetic interactions that promote or sustain tumor formation. Sleeping Beauty（SB） transposoniated insertional mutagenesis has emerged as an exciting approach to identify novel cancer-causing genes in the mouse. The SB transposon faithfully ＂hops＂ throughout the genome by a cut-and-paste mechanism mediated by the ubiquitous expression of the SB transposase. Initial tumor data generated using an SB transposon harboring the MSCV promoter demonstrated a bias towards hematopoietic tumors. More recently, experiments using a modified SB transposon containing the CAG promoter have generated cohorts of mice with solid tumors, primarily carcinomas, which in some cases metastasize. Many animals also develop multiple, inde- pendent primary tumors. These data demonstrate the utility of the SB transposition system for cancer gene discovery across organ systems.
Cancer gene discovery continues to drive current cancer research with the promise of identifying new diagnostic markers and therapeutic targets by elucidating novel genetic interactions that promote or sustain tumor formation. Sleeping Beauty（SB） transposoniated insertional mutagenesis has emerged as an exciting approach to identify novel cancer-causing genes in the mouse. The SB transposon faithfully ＂hops＂ throughout the genome by a cut-and-paste mechanism mediated by the ubiquitous expression of the SB transposase. Initial tumor data generated using an SB transposon harboring the MSCV promoter demonstrated a bias towards hematopoietic tumors. More recently, experiments using a modified SB transposon containing the CAG promoter have generated cohorts of mice with solid tumors, primarily carcinomas, which in some cases metastasize. Many animals also develop multiple, inde- pendent primary tumors. These data demonstrate the utility of the SB transposition system for cancer gene discovery across organ systems.
