Survivin基因在肝门部胆管癌组织中的表达及其与nm23和PTEN相关性的研究

Expression of Survivin and its correlation with PTEN and nm23 in hilar cholangiocarcinoma

目的:探讨Survivin基因在肝门部胆管癌组织中的表达情况以及与nm23和PTEN基因表达的相关性。方法:应用免疫组织化学方法测定肝门部胆管癌及相应癌旁正常组织中Survivin、nm23和PTEN基因的表达,分析Survivin基因表达与肿瘤组织病理分级、淋巴结转移以及nm23和PTEN基因的相关性。结果:44例肝门部胆管癌组织中,Survivin基因阳性表达率63.6%,15例癌旁组织中Sur-vivin基因阳性表达率为6.67%,二者比较差异有统计学意义(P<0.05);Survivin基因表达与病理分级无关(P>0.05),与淋巴结转移无关(P>0.05);Survivin基因表达与PTEN及nm23的表达呈负相关(P<0.05)。结论:Survivin基因的高表达在肝门部胆管癌的发生、进展和转移过程中可能起重要作用;联合检测Survivin与PTEN及nm23基因表达可能有助于临床诊断及判断预后。

Objective： Investigate the expression of Survivin and its correlation with the expression of PTEN and nm23 in hilar cholangiocarcinoma.Methods：In 44 specimens of hilar cholangiocarcinoma, 15 specimens of tissues near the carcinoma （the distance to the edge of tumor ≥ 1 centimeter and no infiltration pathologically）, the expressions of Survivin, PTEN and nm23 were detected by immunohistochemistry methods. Laboratory data were then analyzed statistically together with the pathological data. Results： In 44 cases of hilar cholangiocarcinoma, the positive rate of Survivin was 63.6%, in tissues near the carcinoma the positive rate was 6.67% ;The expression of Survivin in hilar cholangiocarcinoma had no association with pathological stage and lymph node metastasis （P〉 0.05）; The expression of Survivin in hilar cholangiocarcinoma was closely statistically related with the expressions of PTEN and nm23. Conclusion： The expression of Survivin in hilar cholangiocarcinoma is significantly higher than that in tissues near carcinoma, suggesting its correlation with tumor; Survivin plays an important role in the oncogenesis and metastasis of hilar cholangiocarcinoma; Survivin is statistically related with PTEN and nm23 and detected together may helpful for diagnosis and prognosis of hilar cholangiocarcinoma.