摘要
目的:构建以减毒沙门氏菌为载体的小鼠肝炎病毒DNA疫苗,研究该疫苗的免疫原性。方法:以小鼠肝炎病毒S1基因的重组真核表达质粒pVAX1-S1免疫BALB/c小鼠,ELISA检测其诱导抗体产生情况;再将重组质粒pVAX1-S1电转化到减毒鼠伤寒沙门氏菌SL7207中,构建运送S1基因的重组减毒沙门氏菌SL7207(pVAX1-S1),口服免疫BALB/c小鼠,间接免疫荧光试验鉴定减毒沙门氏菌运送的DNA疫苗的免疫原性。结果:与pVAX1空载体对照组相比,重组真核表达质粒pVAX1-S1免疫组二免及三免后抗体水平分别存在显著性差异(P<0.05)和极显著性差异(P<0.01)。减毒沙门氏菌运送的DNA疫苗SL7207(pVAX1-S1)诱导小鼠产生了特异性的血清抗体。结论:构建的重组减毒沙门氏菌SL7207(pVAX1-S1)具有良好的免疫原性,可诱导小鼠产生特异性的体液免疫应答。这为进一步研制冠状病毒新型基因疫苗奠定了基础。
Objective： To construct attenuated Salmonella delivering mouse hepatitis virus DNA vaccine and to study its immunogenicity. Methods： 6-week-old female BALB/c mice were immunized intramuscularly（i.m.） with recombinant plasmid pVAX1-S1 at 2-week intervals for a total of three injections. The humoral immune response was evaluated by ELISA. The recombinant plasmid pVAX1-S1 were then transformed by electroporation into attenuated Salmonella typhimurium strain SL7207. The immunogenicity of attenuated Salmonella delivering mouse hepatitis virus DNA vaccine were detected with indirect immunofluorescent assay. Results： The levels of serum antibody were significantly higher in the mice of pVAX1-S1 groups than that of the negative control group at 2 week post-priming（P〈0.05） and at 3 week post-boosting （P〈0.01）. Serum antibody in mice immunized with attenuated Salmonella delivering mouse hepatitis virus DNA vaccine were successfully detected with indirect immunofluorescent assay. Conclusion： The constructed recombinant Salmonella SL7207（pVAX1-S1） has strong immunogenicity, and could induce humoral immune response in immunized mice. These results lay foundation for developing the coruovirus gene engineering vaccine.
出处
《生物技术通讯》
CAS
2007年第6期912-914,共3页
Letters in Biotechnology
基金
国家自然科学基金项目(30425031)
江苏省重点实验室开放课题(02738960314)
