摘要
目的:研究肝细胞癌(HCC)组织中人白细胞抗原(HLA)Ⅰ类分子轻链β2-微球蛋白(β2-m)在肿瘤免疫逃逸中的作用,探讨肝硬化背景对肝癌组织内β2-m的mRNA水平的影响。方法:RT-PCR法检测40例肝癌和癌旁组织中β2-m的mRNA水平,并从不同角度进行分析。结果:肝癌组织中β2-m基因mRNA水平显著低于非癌组织,肝硬变背景下肝癌组织内β2-m的mRNA水平低于无肝硬化背景的肝癌组织。结论:肝癌组织中β2-m基因mRNA水平下调,可致肝癌细胞表面HLA-Ⅰ类分子低表达,这一变化与肿瘤细胞的免疫逃逸相关;HCC病人的肝硬变背景在这一机制中可能起到了某种程度的促进作用。
Objective: To analyze the mRNA level of β2-microgloblin(β2-m) in hepatocellular carcinoma(HCC) to serve as an evidence that tumor cells can reduce their immunogenicity to evade the attack from immunocytes as a result of their lower expression of class I HLA molecule. To investigate if liver cirrhosis can affect the mRNA lever of β2-m in HCC. Methods: The mRNA was extracted from liver cancer and its paracancerous tissue from fourty patients and semiquantitatively analyzed by RT-PCR technique. Results: The mRNA of β2-m in HCC was significantly lower than that of patched paracancerous tissue and showed less level in tissue with liver cirrhosis than without. Conclusion: Down-regulated β2-m gene led to unstability and incapacity of HLA complex and then made tumor cells escape from body immunal surveillance. The patients' cirrhosis backgrounds have somewhat played as a promoter in tumor escape mechanism.
出处
《生物技术通讯》
CAS
2007年第6期921-923,共3页
Letters in Biotechnology
基金
天津市科委攻关项目(05YFSZSF02500)
科技创新能力与体系建设平台项目(05SYSYJC02600)