几种不同碳、氮源对隐甲藻生长及二十二碳六烯酸产量的影响

Effects of Carbon and Nitrogen Sources on Growth and DHA Yield in Crypthecodinium cohnii

目的:找出有利于隐甲藻生长和二十二碳六烯酸(DHA)积累的碳、氮源。方法:利用不同碳、氮源培养隐甲藻,收集藻体后提取脂肪酸并甲酯化,然后利用毛细管气相色谱法进行分析。结果:最适的单一碳、氮源分别为葡萄糖、酵母粉,在此培养条件下隐甲藻培养72 h后的生物量(干重)和DHA产量分别为3.90和0.642g/L。结论:葡萄糖、酵母粉分别作为碳、氮源时更有利于隐甲藻的生长和DHA的积累。

Objective： To find out the optimum carbon and nitrogen sources which were beneficial to growth and DHA accumulation of Crypthecodinium cohnii. Methods： After cultivating C.cohnii with different carbon and nitrogen sources, cells were collected. Then the fat acids were extracted. After methylating the fatty acid, it was analyzed by capillary gas chromatographic（CGC ） methods. Results ＆ Conclusion： Glucose was best single carbon source and yeast extract was best single nitrogen source for growth and DHA accumulation of C.cohnii. At this value, the productivity of biomass reached 3.9 g/L（dry weight） and the yied of DHA achieved 0.642 g/L after culturing 72 h.