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几种不同碳、氮源对隐甲藻生长及二十二碳六烯酸产量的影响 被引量:3

Effects of Carbon and Nitrogen Sources on Growth and DHA Yield in Crypthecodinium cohnii
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摘要 目的:找出有利于隐甲藻生长和二十二碳六烯酸(DHA)积累的碳、氮源。方法:利用不同碳、氮源培养隐甲藻,收集藻体后提取脂肪酸并甲酯化,然后利用毛细管气相色谱法进行分析。结果:最适的单一碳、氮源分别为葡萄糖、酵母粉,在此培养条件下隐甲藻培养72 h后的生物量(干重)和DHA产量分别为3.90和0.642g/L。结论:葡萄糖、酵母粉分别作为碳、氮源时更有利于隐甲藻的生长和DHA的积累。 Objective: To find out the optimum carbon and nitrogen sources which were beneficial to growth and DHA accumulation of Crypthecodinium cohnii. Methods: After cultivating C.cohnii with different carbon and nitrogen sources, cells were collected. Then the fat acids were extracted. After methylating the fatty acid, it was analyzed by capillary gas chromatographic(CGC ) methods. Results & Conclusion: Glucose was best single carbon source and yeast extract was best single nitrogen source for growth and DHA accumulation of C.cohnii. At this value, the productivity of biomass reached 3.9 g/L(dry weight) and the yied of DHA achieved 0.642 g/L after culturing 72 h.
出处 《生物技术通讯》 CAS 2007年第6期968-970,共3页 Letters in Biotechnology
关键词 隐甲藻 碳源 氮源 二十二碳六烯酸 Crypthecodinium cohnii carbon source nitrogen source DHA
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同被引文献42

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