摘要
链霉菌φC31噬菌体整合酶(φC31-int)属于位点特异性重组酶的解离酶/转化酶系,该家族催化机制由丝氨酸介导,能识别噬菌体附着位点(attP)和宿主基因组上的细菌附着位点(attB),介导同源序列之间的位点特异性重组。实验证实,φC31-int是一种高效位点特异性整合工具酶,与其他整合策略相比,具有集高效和安全于一体的优点,通过介导位点特异性整合,将外源基因特异地整合到宿主基因组,使目的基因得以持续、高效的表达,并且具有DNA容量方面的优势。随着研究的深入,人们对φC31-int介导整合作用机制和影响因素有了进一步的认识。通过一系列成功应用φC31-int进行的基因治疗的动物学实验,为今后如何利用非病毒载体系统进行基因治疗及转基因动物模型的建立提供了一种新的选择。
φC31 integrase（φC31-int） belongs to the serine recombinase family that can catalyses the recombination reaction between the phage attachment site（attP） and the bacterial attachment site（attB）. Studies prove that φC31-int is a valuable site-special integration tool enzyme because of its efficiency and safety. This integrase can perform an site-special chromosomal integration of aimed DNA into host genomes that results in long-term and efficient transgene expression, which also has the DNA capacity dominance. With further study, researchers know more about the mechanism and influ- ence factors on the integration reaction mediated by φC31-int and serial successful animal gene therapy studies by using φC31-int suggest the possible utility of nonvirus vectors as φC31-int for gene therapy and constructing transgene animal model.
出处
《生物技术通讯》
CAS
2007年第6期1036-1038,共3页
Letters in Biotechnology
