离子液体微波辅助萃取石蒜中生物碱的研究

Microwave-assisted Extraction of Alkaloids in Lycoris Radiata Using Ionic Liquids Solution

以离子液体溶液为溶剂,研究了离子液体微波辅助萃取石蒜中石蒜碱、力克拉敏和加兰他敏生物碱。考察了离子液体的种类和浓度对萃取石蒜生物碱的影响,并与传统有机溶剂和传统萃取技术进行了对比研究。结果表明,1.0mol/L[bmim]Cl溶液为溶剂,液固比(mL∶g)15∶1,80℃微波辅助萃取10min,石蒜碱、力可拉敏和加兰他敏生物碱的萃取率分别为2.730、0.857和0.179mg/g。与传统的萃取方法比较,本方法快速高效、环境友好。

The application of ionic liquids aqueous solutions as solvents in the microwave-assisted extraction （MAE） technique was developed for the extraction of lycorine, lycoramine and galanthamine from Lycoris. Effects of different ionic liquids and the concentration of selected ionic liquids on MAE were investigated, and tradition organic solvent and extraction techniques were compared. The optimal MAE conditions were that the solvent was 1.5 mol/L 1-buthyl-3-methylimidazolium chloride [ bmim] Cl solution, the ratio of liquid/solid （mL： g） was 15：1, the extraction temperature was 80℃ and time was 10 min. Under these MAE conditions, the yields of lycorine, lycoramine, galanthamine were 2. 730, 0. 857 and 0. 179 mg/g respectively. This proposed method was a good, rapid and environmentally-friendly alternative technique to extract and alkaloids in lycoris as compared with traditional extraction technique.