反相高效液相色谱法测定丙酸发酵液中的有机酸

Determination of Organic Acids in Propionic acid Fermentation Broth by Reversed Phase High Performance Liquid Chromatography

对于生物法制备丙酸的微生物发酵体系，利用Waters Atlantis dC18反相色谱柱，以0．01mol／LKH2PO4缓冲液（pH2．8）-乙腈（95：5，V/V）作流动相，在流速为1．0mL／min，柱温为25℃时，于紫外波长215nm处检测，9min内发酵液中的草酸、甲酸、乙酸、乳酸、琥珀酸和丙酸能得到良好分离。6种有机酸线性相关系数均在0．9992-1．0000之间；方法的回收率为99％～102％；RSD为0．56～1．66。本方法能够简便、快速测定丙酸发酵体系中主产物丙酸与其它有机酸含量，适于指导整个发酵过程条件的优化及发酵产物的监控。

Water altanlis with dC18 column and mobile phase contained 10 mmol/L potassium dihydrogen phosphate buffer （pH 2.8） and acetonitrile （95：5, V/V） was used to determin organic acid in propioic acid fermenlation system. The flow rate was 1.00 mL/min and the column temperature was 25℃. Oxalic acid, formic acid, lactic acid, acetic acid, succinic acid and propionic acid were completely separated and determined with UV detector at 215 nm in 9 min. All the correlation coefficients（r） were between 0.9992 and 1. 0000, and the recovery ranged from 99% to 102%. The relative standard deviation was in the range of 0.6% - 1.7%. By this method, propionic acid and other organic acids could be rapidly and effectively determined.