杜氏利什曼原虫无鞭毛体蛋白基因真核表达重组质粒构建

Construction of Eucaryotic Expression Recombinant Plasmid of Amastin Gene of Leishmania donovani

目的构建杜氏利什曼原虫无鞭毛体蛋白(amastin)编码基因的真核表达重组质粒pcDNA3.1-amastin。方法提取杜氏利什曼原虫基因组DNA进行PCR扩增,将扩增出的无鞭毛体蛋白基因片段导入质粒载体pcDNA3.1(+),构建真核表达重组质粒pcDNA3.1-amastin。结果扩增出大小约550bp的无鞭毛体蛋白基因;重组质粒pcDNA3.1-amastin经鉴定正确。结论成功构建杜氏利什曼原虫无鞭毛体蛋白基因真核表达重组质粒pcDNA3.1-amastin。

Objective To construct eukaryotic expression recombinant plasmid of amastin gene of Leishmania donovani. Method Amastin gene was amplified from nuclear DNA of Leishmania donovani isolates and cloned into an eukaryotic expression vector pcDNA3.1 （ ＋ ）. The recombinant plasmid was named pcDNA3.1-amastin. Result Amastin gene of 550bp was acquired by PCR. The recombinant plasmid pcDNA3.1-amastin was correct by analysis. Conclusion The eukaryotic expression recombinant plasmid of amastin gene of Leishmania donovani was constructed successfully.