RP-HPLC法测定舟车丸中大黄素和大黄酚含量

Determination of Emodlin and Chrysophanol in Zhouchewan Pills by RP-HPLC

目的：测定舟车丸中大黄素和大黄酚含量。方法：采用RP-HPLC法测定舟车丸中大黄素和大黄酚含量。色谱柱：心phere ODS（4．6mm×250mm，2．5μm），流动相：乙腈-0．1％磷酸（70：30），检测波长：254nm，流速：1mL/min，进样量：10μL。结果：大黄素和大黄酚分别在0．0524—0．2620和0．1228—0．6140μg范围内线性关系良好，其平均回收率分别为96．70％和97．33％，制剂中其他味药材对含量测定无干扰。结论：该方法准确、操作简单、专属性和重现性良好，可以有效用于舟车丸的质量控制。

Objective： To determine emodin and chrysophanol in Zhouchewan pills. Methods： Emodin and chrysophanol in Zhouchewan pills were determined by RP-HPLC. An Allsphere ODS column （4.6 mm× 250 mm, 2.5 μm） and acetonitrile-0.1% phosphoric acid （70：30） as the mobile phase were used at a flow rate of 1 mL/min, with a detection wavelength of 254 nm and an injection volume of 10 μL. Results： For emodin and chrysophanol, the linear ranges were 0.052 2 - 0.062 0 and 0. 122 8 - 0.613 8 μg respectively; the average recoveries were 96.58% and 97.33% respectively. Other ingredients in the pills had no interference with the determination. Conclusion： The method is acurate, simple, specialized and reproducible. It is suitable for quality control of Zhouchewan pills.