微生物降解法处理含酚废水

The Utilazation Study on the Microbial Degradation of Phenol Waste

目的筛选苯酚降解菌并研究固定化苯酚降解菌对含酚废水的降解效率,为利用微生物降解法处理含酚废水奠定基础.方法采用平皿稀释分离法在筛选平板中分离出苯酚降解菌,并进一步通过驯化及固定化手段提高菌株的降酚率.结果筛选出一株苯酚降解菌S10;经进一步驯化及固定于聚乙烯醇凝胶颗粒后,在实验条件下对质量浓度为1 000 mg/L的含酚废水的降解率达到90%以上,且固定化细胞对环境的pH、温度的耐受能力以及对热的稳定性等性能增强结论采用固定化降酚菌株的方法处理含酚废水不仅具有较高的降解能力而且固定化细胞更能适应多变的环境条件.

Microbial degradation of phenol waste, a simple and efficient method, has more unexampled merits than the other traditional methods. By experiment, Strain S10 was obtained from the soil polluted by petroleum. When immobilized with PVA, the activity of strain S10 degradating phenol waste would be about 90 %. Moreover, the properties of immobilized cells would change greatly：The optimum temperature and pH of free cells were 30℃ and 7.0 respectively, and no more than 10 % degradation activity was remained when it had been kept at 80℃ for 20 minutes. While, if the strain S10 was immobilized with PVA, the optimum temperature and pH were 40℃ and 7.0 respectively, and the degradation activity was more than 50% after treated at 80℃ for 120 minutes. It could be anticipated that microbial degradation of phenol waste has a broad future.