液相色谱-质谱法同时鉴别中药制剂中的15种糖皮质激素

Simultaneous Identification of Fifteen Glucocorticoids in Chinese Traditional Medicine Preparations by Liquid Chromatography-Mass Spectrometry

为了打击药物的非法滥用,建立起鉴别中药制剂中可能添加的糖皮质激素的液相色谱-质谱分析方法。针对不同的中药剂型,采用不同的试样处理方式。提取后的样品溶液采用Diamond C18柱(5μm,250mm×4.6mm)分离,以流动相A(水-四氢呋喃,体积比为100∶1)和B(乙腈-水-四氢呋喃,体积比为80∶20∶1)进行梯度洗脱。通过与对照品保留时间、一级质谱及二级质谱图的对比进行定性。应用该法鉴定了中药制剂中的糖皮质激素,在供试品溶液中检出了醋酸泼尼松。

To monitoring illegal abuse of these drugs, a liquid chromatography-mass spectrometry （LC-MS） method for identification of glucocorticoids in Chinese traditional medicine preparations was developed. Samples were extracted using different methods according to the nature of products. The analyses were performed on a Diamond C18 column （5 μm, 250 mm×4.6 mm） with gradient elution using water-tetrahydrofuran （100： 1, v/v） as mobile phase A and acetonitrile-water-tetrahydrofuran （80： 20： 1, v/v） as mobile phase B. Prednisone acetate in the preparation used to cure dermatitis was identified by comparing the retention time, full scan MS, and full scan MS^2.