NF-κB在腹部肠管火器伤后肝组织中的表达及意义

Expression of NF-kB and Its Significance in Liver Injury after Intestinal Perforations Due to Abdominal Firearm Wound

目的:探讨NF-κB在腹部火器伤肠管穿透后肝组织中的变化及意义。方法:42头健康长白仔猪随机等分为对照组和伤后1h、2h、4h、8h、12h和24h组,实验组建立腹部肠管火器伤模型后,用免疫组化图像分析法测定各组肝内NF-κB的表达,同时测定血清中ALT、AST水平,光镜下观察各组肝脏组织学变化。结果:实验组肝内NF-κB活性明显高于对照组,并于伤后1h和8h出现2个高峰(P<0.05)。实验组出现逐渐加重的肝细胞水肿、变性、坏死,血清ALT、AST水平在伤后明显升高,并于伤后2h和12h出现2个高峰(P<0.05)。结论:NF-κB的活化在腹部火器伤肠管穿透后继发性肝损伤中扮演着重要角色。

Objective： To investigate the expressions of NF-KB and its significance in liver injury after intestinal perforations due to abdominal firearm wound. Methods： 42 Changbai piglets were randomized into 7 groups： control group and wounded 1 hour group, 2 hour group, 4 hour group, 8 hour group, 12 hour group, 24 hour group. The model of intestinal perforations due to abdominal firearm wound was established in wounded groups. Hepatic NF-KB expression was measured with immunohistochemistry and image analysis in all groups. Levels of serum ALT and AST were determined. The changes of hepatic tissue were observed under light microscope in all groups. Results： Levels of hepatic NF-KB expression in wounded groups were significantly elevated compared to control group, and they appeared two peaks in lh group and 8h group （P〈0.05）. Hepatocyte edema,metamorphosis and necrosis was gradually aggravated from lh group to 24h group, Levels of serum ALT and AST were all markedly increased, and they appeared two peaks in 2h group and 12h group （P〈0.05）. Conclusion： The activation of NF-KB play an important role in secondary liver injury after intestinal perforations due to abdominal firearm wound.