畜禽废水胁迫对芦苇叶绿素含量及抗氧化酶系统的影响

Effects of Livestock Wastewater on the Content of Chlorophyll and Antioxidant Enzyme Systems in Leaves of Phragmites australis

采用自然条件模拟实验,以两种浓度畜禽废水(高浓度畜禽废水原水和经一倍稀释的中浓度畜禽废水)胁迫湿地植物芦苇,研究其对芦苇叶绿素含量及其抗氧化酶系统的影响。结果表明,高浓度畜禽废水较中浓度畜禽废水对芦苇的影响更大,两种浓度畜禽废水胁迫均促使芦苇叶绿素含量下降、叶绿素a/b值上升、CAT活性下降、POD活性急速下降后逐渐上升,到处理末期时与对照基本相同、SOD活性明显上升。说明畜禽废水胁迫对芦苇的影响较小,芦苇在畜禽废水胁迫下具有较强的抗逆性和耐受性,如果将其运用于我国集约化畜禽养殖业废水的处理,将极大地缓解我国畜禽养殖业发展与环境污染之间的矛盾。

Effects of livestock wastewater treatment on the chlorophyll and antioxidant enzyme systems in leaves of Phragrnites australis were investigated by simulating the natural condition under two concentrations of livestock wastewater： high concentration of raw livestock wastewater and mid-concentration of livestock wastewater which was diluted to half from the raw livestock wastewater. High concentration livestock wastewater had stronger effect on Phragmites australis than mid-concentration livestock wastewater. Under the stress of livestock wastewater, the content of chlorophyll decreased, the ratio of chlorophyll a/b increased, the activities of catalase （CAT） decreased, the activities of peroxidase （POD） decreased obviously at the first three days and then reached almost the same activities at the end of the treatment when compared with the control, while the activities of superoxide dismutase （SOD） increased but fluctuated during the treatment. The results indicated that Phragmites australis had very strong anti-adversity abilities and tolerances to livestock wastewater, and could enormously relieve the contradiction between the development of livestock breeding and environment contamination if it was used in the treatment of livestock breeding wastewater.