促红细胞生成素对大鼠脑外伤脑组织线粒体ATP酶活性的影响

Effect of recombinant human erythropoietin on activity of ATPase of brain mitochondria after brain injury in rats

目的观察体外注射重组人促红细胞生成素(rhEPO)对大鼠脑外伤脑组织线粒体ATP酶活性的影响,探讨rhEPO对脑外伤后神经保护作用的机制。方法建立大鼠自由落体脑挫裂伤模型,伤后立即腹腔注射rhEPO,采用改良Lowry氏法分别测定治疗后4、12、24和48 h及各自对照组大鼠脑组织线粒体Na+-K+ATP酶、Ca2+-ATP酶及Mg2+-ATP酶活性。结果脑外伤后大鼠脑组织线粒体Na+-K+ATP酶、Ca2+-ATP酶及Mg2+-ATP酶活性均显著下降(P<0.05)。rhEPO治疗后12、24和48 h脑组织线粒体ATP酶活性均显著高于各自时间点对照组(P<0.05)。结论外源性rhEPO可通过影响线粒体功能而减轻脑外伤后的继发性脑损害,从而改善预后。

Objective To explore the effect of recombinant human erythropoietin （rhEPO） on activity of ATPase of brain mitochondria after brain injury in rats, and elucidate the possible mechanism of the neuroprotective effect of rhEPO administered to rats with brain injury. Methods The models of laceration of brain included by free falling were established, rhEPO was intraperitoneally injected immediately after brain injury. The mitochondria were isolated at 4 h, 12 h, 24 h, 48 h respectively after treatment, and the levels of activity of Na ^＋ -K^＋ ATPase, Ca^2＋ -ATPase and Mg^2＋ -ATPase of brain mitochondria in the control and rhEPO group were measured by modified Lowry method. Results The levels of activity of the mitochondria Na ^＋ -K^＋ ATPase, Ca^2＋ -ATPase and Mg^2＋ -ATPase were significantly decreased in rats with brain injury（ P 〈0.05） , while significantly elevated 12 h, 24 h and 48 h after treatment with rhEPO compared with the corresponding control group （ P 〈 0.05）. Conclusion Intraperitoneal administration with rhEPO can impove the activity of motochondria ATPase after brain injury in rats, therefore the secondary damage to the brain can be relieved and the prognosis of the brain injury and be improved.