微球状骨修复材料研究

Microspheres as substrate materials for bone repair

结合自体碎骨修复和组织工程的原理，在微球材料表面黏附足够的成骨细胞后，植入体内进行骨修复，是骨修复方法中的新思路。选择壳聚糖/β-TCP复合微球以及β-TCP微球作为材料体系。首先采用反相乳液悬浮法制备壳聚糖/β-TCP复合微球，再经过脱脂-烧结工艺获得仅含有B-TCP的无机微球。粒度分析发现，获得的微球球径主要分布在150-450μm范围内，平均球径约300μm。XRD结果显示烧结后的微球中仅有β-TCP相.SEM观察和压汞法测量发现，经烧结后微球表面无尖锐的棱角存在，表面与内部存在大量1．5～5μm的孔隙，孔隙率达到48％以上。复合微球中孔隙较少，其表面较粗糙。细胞培养实验发现细胞在两种微球表面均生长良好，表明它们均具有良好的生物活性和细胞相容性。

Combining the mechanisms of tissue engineering and micro-sized autografts bone reparation, the microspheres with cells on its surface used as bone reparation materials is a good strategy. Chitosar/β-TCP composite microspheres and β-TCP microspheres were selected as the objective materials in this paper. Chitosan/β-TCP composite microspheres was prepared by a solid-in-water-in-oil （s/w/o） emulsion cross-linking method, and then were burn out and sintered to fabricating the β-TCP microspheres. The size of as-fabricated microspheres was mainly distributed from 150μm to 450t.tna, and the average size was about 300μm. Observation by SEM, no spiculate edges and comers on the surface of sintered β-TCP microspheres, and abundant micro pores with diameter of about 1.5-5μm were observed, which was benefit for flowing of body fluid and supply of nutriment and oxygen. The porosity was above 48%, which was measured by mercury porosimetry. For the composite microspheres, although few micro pores were found, the coarse surface was also obtained. Cells cultured experiments showed the cells could and growth on the surface of the two microspheres, and indicted that they possess good bioactivity and cyto-compatibility. Hence, the two microspheres could be used as substrate materials for bone repair.