摘要
在研究纳米羟基磷灰石（Calo（PO4）6（OH）2，HAP）参与生命过程和代谢机制时，其溶解特性研究是至关重要的，它是其纳米生物学效应机理研究的基础。本研究利用磷钼酸铵显色法准确测定HAP纳米粒子在37℃的水中的溶解特性。所得结果显示经高温处理的羟基磷灰石纳米粒子和未经高温处理的HAP纳米粒子的溶解常数对数值pKsp在74左右．明显低于已知的块体羟基磷灰石的pKsp值117．0，甚至还略低于无定形磷灰石，说明纳米羟基磷灰石在溶解特性上表现出与常规块体羟基磷灰石显著的差别，其溶解度或离解度大大高于非纳米的羟基磷灰石，纳米HAP在与肝癌细胞相互作用时表现出的纳米生物学效应可能与它的这种特性有关．
Nano-hydroxyapatite （Ca10（PO4）6（OH）2, HAP） could enter the human liver cancer cells in form of nanoparticles and change the internal environment of cancer cells. The the water solubility properties of nano-hydroxyapatite are the key factors to reveal the mechanism of bioeffects. The phosphate anion concentration was measured by chromogenic reaction of ammonium phosphomolybdate. The obtained concentrations of phosphate are applied to calculate the pKsp value of HAP nanoparticles samples. The pKsp value is about 74. It is significantly lower than the known value 117 of blocky hydroxyapatite. It is even slightly lower than that of amorphous calcium phosphate. That reveals that nano hydroxyapatite has remarkable difference in dissolution properties from blocky hydroxyapatite. The solubility or dissociation degree of nano-HAP is much higher than that of blocky HAP.
出处
《功能材料》
EI
CAS
CSCD
北大核心
2007年第A05期1733-1736,共4页
Journal of Functional Materials
基金
The project supported by National Natural Science Foundation of China （50472040）
Hubei Provincial Science and Technology Committee （2001AA306B02, 2002AA 105A06）
关键词
溶解特性
羟基磷灰石
纳米晶体
紫外分光光度法
solubility properties
hydroxyapatite
nanocrystals
ultraviolet spectrophotornetry
