胶原-壳聚糖二元膜材料的制备与性能表征

The preparation and characterization of collagen-chitosan composite membranes

通过热脱氢交联（DHT）、戊二醛改性以及碳化二亚胺改性（EDC）等方法对胶原基膜材料进行改性，探讨其作为生物医用材料较为理想的改性方法。以胶原及壳聚糖为主要原料制备大孔径高孔率二元膜材料，通过单一或复合改性以后比较二元膜材料的各项性能。适当的DHT改性后获得的二元膜孔径为20～100μm，孔率90％左右，但膜材料机械性能较差，抗酶解能力提高不明显；0．02％的戊二醛．EDC复合改性交联程度较为合适，DHT改性可以一定程度减少戊二醛用量；DHT-EDC复合改性得到的二元膜孔径20～200μm，孔率95％左右，机械强度和耐酶稳定性较高，吸水率高且在水中不溶胀。DHT-EDC复合改性是较为理想的胶原基膜材料作为生物医用材料的改性方法。

The collagen-based membranes were modified by dehydrathermal（DHT）, glutaraldehyde and 1-ethyl-3- （3-dimethylaminopropyl）carbodiimide （EDC）, which were discussed as application in biomaterial modification. Methods： Collagen and chitosan were used as raw materials in preparation of the composited membranes with bigger pore diameter and higher porosity, and aspects of the characters were evaluated after modification by one or composited methods. The composited membranes modified by DHT had the pore diameter of 20-100μm and the porosity was about 90%, but the merchanical properties was relatively poor, as well as the ability of anti-enzymolysis; the degreee of crosslinking was suitable when modified by 0.02% GA-DHT, while the DHT would reduce the dosage of GA in some extent; the pore diameter of the membranes modified by DHT-EDC was 20-200μm, and the porosity was about 95%, with the characteries of ideal merchanical properties, anti-enzymolysis and stability in soultion. The composite method of DHT-EDC is satisfied for the modification of collagen-based materials in tissue engineering.