钛表面层层组装胶原与肝素改善血液相容性的研究

Surface modification of titanium via layer-by-layer self-assembly of collagen and heparin multilayers to improve blood-compatibility

为了改进钛表面的血液相容性，本实验通过电荷作用层层组装带正电的细胞外基质胶原和带负电的肝素，在钛表面形成多层仿生膜，以改善钛表面的血液相容性。钛通过氢氧化钠处理，表面形成多孔结构，并产生碱性羟基，之后将其浸泡于多聚赖氨酸溶液中，然后将材料交替浸泡于肝素和胶原中，最后一层为肝素，通过电荷吸引形成多层膜．通过傅立叶红外漫反射（FTTR）检测各步反应后表面基团的变化，通过扫描电子显微镜（SEM）观察表面形貌的变化，初步说明肝素已经结合在材料表面；血小板黏附实验直观反应组装膜有良好的血液相容性。通过本实验说明这种改性方法对于材料血液相容性的改善有一定价值。

To improve the surface blood-compatibility of titanium, a layer-by-layer （LBL） self- assembly technique, based on the polyelectrolyte-mediated electrostatic adsorption of collagen with positively charged and heparin with nagatively charged, was used leading to the formation of multilayers on the titanium surfaces.The film growth was initialized by deposition of one layer of positively charged poly-L-lysine on titanium surface with nagatively charged treated by NaOH. Then the multilayers thin film was formed by the alternate deposition of negatively charged heparin and positively charged collagen utilizing electrostatic interactions. The LBL film growth was monitored by several techniques. The chemical composition and surface topography were investigated by using fourier transform infrared spectroscopy and scanning electron microscope. Platelets absorded of material surface directly showed LBL-modified titanium films have better anticoagulation performance than control. These datas suggest that col/hep were successfully employed to surface engineer titanium via LBL technique, and enhanced its blood-compatibility.