APTE修饰钛氧膜并固定Ⅰ型胶原对内皮细胞生长的影响

Influence of APTE modification and binding type Ⅰ collagen to TiO2 film on the proliferation of endothelial cells

在用磁控溅射合成具有一定特性的TiO2薄膜的基础上，使用一定浓度的NaOH溶液对其表面进行活化，并通过氨丙基三乙氧基硅烷（APTE）偶联固定I型胶原，通过傅立叶红外光谱（PTIR）、X射线光电子能谱（XPS）等检测手段进行材料处理前后表面性质表征．通过人脐静脉内皮细胞种植试验评价内皮细胞在样品表面的粘附和生长行为．研究结果表明：一定特性的TiO2薄膜经NaOH活化后，表面可产生活性的羟基基团；借助羟基，活化后的TiO2薄膜表面可以与硅烷偶联剂实现偶联固定，产生可以利用的活性氨基基团；进一步地，借助偶联剂的氨基基团，Ⅰ型胶原可以连接到TiO2薄膜表面形成涂层．体外内皮细胞培养实验表明，在处理后的TiO2薄膜表面，内皮细胞的粘附性和生长行为获得改善．因此，通过表面活化后涂覆APTE并固定Ⅰ型胶原修饰医用微弹簧圈，使其在体内更容易实现动脉瘤颈部的内皮化，以使动脉瘤与血液循环完全隔绝．

The surface modification has become a focus in biomaterials research area. In this work, on the basis of our previous study, the TiO2 films were synthesized using Unbalance Magnetron Sputtering （UBMS） to improve its thrombus formation ability at a certain extent. The chosed TiO2 film was pretreated by NaOH solution for activity. Using bio-cherrfical modification method, type Ⅰ collagen was further immobilized on the TiO2 film surface by silane coupling reagent of aminopropyltriethoxysilane （APTE）. X-ray photoelectron spectroscopy （XPS） and fourier transform infrared spectroscopy （PFIR） were used to investigate the characteristics of the bio-chemical modificated TiO2 film and the controlled TiO2 film. The biological behavior of cultured human umbilical vein endothelial cells （HUVECs） onto different films was investigated by in vitro HUVECs cultured experiment. The results showed that the bio-chemical modification can meet the biocompatibility requirement of TiO2 film for biomedical microcoil application to hemangioma treatment.