制备工艺对BSA-PHB／PEG微球性能的影响

Effect of processing conditions on the properties of BSA-PHB/PEG microspheres

以聚3-羟基丁酸酯（PHB）和聚乙二醇（PEG）共混物为原料，引入牛血清白蛋白（BSA）作为模型药物，采用复乳化溶剂蒸发法制备包埋BSA的PHB／PEG微球，研究了制备工艺对微球性能的影响．实验结果表明，较优化的微球制备工艺为：W1／O=1／5，O／W2=1／5，聚合物浓度为4％w／v，配比m（PI-IB）：m（PEG）=4：1；该工艺所得微球的平均粒径在10μm左右，BSA突释率为17．8％，30d的累积溶出量达70％左右，释放特性比较理想．

Microspheres containing bovine serum albumin （BSA, a model drug） were prepared via double emulsion solvent evaporation method using compound of poly（3-hydroxybutyrate） （PHB） and polyethylene glycol （PEG） as the matrix. Effect of processing conditions on the properties of BSA-PHB/PEG microspheres were studied. The results show that improved processing conditions involve W1/O= 1/5 and O/WE=1/5. The concentration of polymer compound is 4% w/v with a 4 ： 1 mass ratio of PHB and PEG. The mean particle size of the microspheres prepared under this conditions was about 10μm. It had a good release characteristic in vitro. Initial burst of BSA was 17.8% and accumulative BSA released after 30 d reached 70% around.