2-甲氧雌二醇对HL60白血病细胞增殖和凋亡的影响

Effect of 2-methoxyestradiol on the proliferation and apoptosis of HL60 cells in vitro

目的探讨2-甲氧雌二醇对HL60白血病细胞增殖及凋亡的影响。方法运用四唑蓝（MTT）法和彗星电泳法检测2-甲氧雌二醇对HL60白血病细胞的增殖抑制及促凋亡作用；应用流式细胞技术检测Bcl-2蛋白的表达。结果（1）2-甲氧雌二醇在10～50μM浓度范围内作用72h以及30μM2-甲氧雌二醇在作用24、48、72h后对HL60白血病细胞有增殖抑制作用，这种作用呈时间-剂量依赖关系。（2）2-甲氧雌二醇在作用浓度为10、30、50μM时，HL60白血病细胞凋亡率分别为8．20％、28．20％、45．30％，与阴性对照组（1．00％）差异有统计学意义；30μM2-甲氧雌二醇在作用24、48、72h后，HL60白血病细胞凋亡率分别为3．71％、17．72％、43．58％，48、72h作用时间组与阴性对照组（1．00％）差异有统计学意义。（3）30μM2-甲氧雌二醇作用72h后的细胞，Bcl-2蛋白的表达较对照组显著减少。结论2-甲氧雌二醇具有对HL60白血病细胞增殖抑制及促凋亡作用，Bcl-2蛋白表达下调在此过程中可能发挥着重要的作用。

Objective To investigate the effect of 2-methoxyestradiol on the proliferation and apoptosis of HL60 cells in vitro. Methods After treated with 2-methoxyestradiol, the cell proliferation was detected with MTT, and apoptosis was measured with comet assay. Expression of Bcl-2 protein in HL60 cell was detected with flow cytometry. Results After treatment with various concentrations （10, 30, 50 μM） of 2-methoxyestradiol for 72h or 30 μM for different time-points （24, 48, 72 h ）, the growth inhibition rate exhibited significant increase （P〈0. 05） in time-dose dependent manner; and the apoptotic rate accounted for 8.20%, 28.20%, 45.30%, 3.71%, 17.72%, and 45.38% respectively, Bcl-2 protein decreased significantly in 30 μM 2-methoxyestradiol 72 h group compared with that of control group. Conclusion 2-methoxyestradiol can inhibit proliferation and induce apoptosis of HL60 in vitro; down regulation of Bcl-2 protein may play an important role in the procedure.